Effects Of Laminarin 01 (L01) On Inhibiting The Thrombus Formation With Injured Endothelial Cells And Ultrastructural Organization Of Cultured Cell

Objective:TO investigate the mechanism of laminaran in inhibiting the thrombus formation according to the establishment of two rat models with injured endothelial cells,including the effect on ultrastructural organization of cultured endothelial cells,thus providing an experimental basis for developing a new drug for antithrombosis.Methods:(1)L01 effect on Thrombus formation in the artery-vein circuit rat models in vivo.The experiment adopts the model of endothelial cells injured by adrenaline.Rats were divided into 7 groups randomly.NS control rats were received normal saline 1d,2d,3d,two times a day.H-L01 treatment rats and L-L01 treatment rats were injected L01 50mg/kg and 10mg/kg respectively, and treated with adrenalin in an hour.Correspondingly,Asp treatment rats were instead of H-Asp,M-Asp and L-Asp.Model rats were injected adrenalin 1d,2d,3d,three times a day.Every group were injected adrenalin before experiment in the fourth day.the weight and the inhibition ratio of thrombus were measured in the artery-vein circuit rat models.(2)Effect on Thrombus formation in the mesentery minicirculation models in vivo.Rats were divided into 6 groups randomly.NS control rats were received normal saline.Model rats were injected endotoxin.L01 treatment rats were injected L01 after being injected endotoxin.Asp treatment rats is the same as L01 treatment rats. Thrombus formation time and blood flow rate in veins were measured in the mesentery minicirculation models.(3)Effect on ultrastructural organization of endothelia cells in vitro.H-L01 treatment,L-L01 treatment,model control,L01 control and normal control were administered in vitro for 48 hours.To observe ultrastructural organization of endothelia cells and damage of the cell organ through TEM.Results:(1)L01 effect on Thrombus formation in the artery-vein circuit rat models in vivo.In the models by injected adrenaline,compared with control group,the weight of thrombus increased significantly.But compared with model group,H-L01(50mg/kg)group and L-L01(10mg/kg)group can decrease the weight of thrombus,and the inhibition ratio in H-L01 group can reach to 57.5%. (2)Effect on Thrombus formation in the mesentery minicirculation models in vivo.In the models by injected endotoxin,compared with control group,thrombus formation time increased.And compared with model group,H-L01(5mg/kg)group and L-L01(2.5mg/kg)group can prolong the thrombus formation time,and on the 1h,the blood flow rate in veins is much higher than model group's.(3)Effect on ultrastructural organization of endothelia cells in vitro.In the models by injected adrenaline,irregularity cellular nucleus,cello-tight junction,microvilli is to decrease and to disaapear. Engorgement of mitochondrium,cristae is to dissolve and disaapear, cytolysosome is to increase.L01 control group and H-L01group is almost the same as the nomal control group;the damage in L-L01group is not as severe as the model group.Conclusions:Laminaria-polysaccharide can inhibit the thrombus formation in two rat models with injured endothelial cells.And improve the damage from the ultrastructural organization of endothelia cells...