Effects Of B7-H4 Gene Modified Islet Cells On The Allograft Rejection In Islet Transplantation

In this study, The recombinant expression vector, rAAV-mB7H4-hrGFP, was constructed and prepared to transfect islet cells which were isolated and purified from BALB/c mouse pancreases. Then C57BL/6 mice were administered with STZ through peritoneal injection to make the mouse model of diabetes. These diabetes mice were transplanted with mB7-H4-islets, hrGFP-islet, and normal islet. The effects of islet cells modified with mB7-H4 before transplantation on the rejection of murine islet allograft were investigated. The results verify that mB7-H4 modified islet allograft can prolong the survival of grafts significantly and induce the hyporesponsiveness of sensitized T cells. This study supplies a new experimental proof for using B7-H4 gene modified islet cells to prevent the islet allograft rejection which is an obstacle in the islet transplantation for treatment of IDDM. Therefore, the intervention measures based on negative co-stimulatory molecules may have significant theoretic and applicative values in the treatment of deseases.