Study On The Treatment Of Diabetes By Islet Transplantation At Omentum Microenvironment

At present,there are 425 million diabetics in the world,and the number of patients is rapidly increasing.It is estimated that by 2045,there will be nearly 700 million people with diabetes.Diabetes is a metabolic disease characterized by hyperglycemia,and its complications are as many as 100.It is currently the most known complication,and drug treatment is difficult to reverse.The complications of diabetes resulted in morbidity and mortality which represents a significant health care burden to the family and the country.Islet transplantation has been shown to be only therapeutic option which can restore entirely normal glucose homeostasis.The selection of islet transplantation site and construction of the in vivo environment suitable for islet survival are the key issues and research hotspot for islet transplantation.In this study,a mouse model of type I diabetes mellitus(TIDM)was established to observe changes in blood glucose and islet function before and after islet transplantation of the same mouse,and to examine the effectiveness of islet cell isolation,purification,and transplantation.To investigate whether the omentum is an ideal site for transplantation,we compared the blood glucose level and survival status after islet transplantation at different transplant sites(omentum and kidney),and the specific research content and results are as follows:(1)The collagenase was perfused into the pancreas of the mouse by common bile duct puncture and it was found that the pancreas was completely inflated and the perfusion was good.Subsequently,the pancreas was excised and shake-digested at a suitable temperature.The pancreas was digested into fine sand grains and digested completely;(2)The pancreatic islets were purified by density gradient centrifugation and stained with dithizone(DTZ).Islets were observed to be scarlet-like,round or oval under an inverted phase contrast microscope.After the manual picking of the islets under a stereo microscope,high purity islets were finally obtained;(3)After the insulin release test and cell activity assay,it was found that the cultured islet cells all showed good activity and could be used for further transplantation;(4)Glucose meter was used to test the blood glucose levels of the mouse model of diabetes after the omentum and kidney transplantation.It was found that the blood sugar level of the diabetic mice after transplantation began to stabilize and maintain normal level after two weeks.Mean blood glucose levels before and after membrane transplantation were similar to those in the kidney group;(5)Using the random effects model to analyze the interaction of time-group factors,the results showed that there was no significant difference between the two groups of blood glucose values and time;(6)The histological observation of islet cells at different transplant sites survived well without inflammatory cell infiltration.In summary,compared with the kidney,the omentum is a safe and convenient site for transplantation.It not only provides sufficient space for islets,has advantages in blood supply,but also has suitable islet growth environment.Therefore,it can be used as an ideal transplant site for future studies,but the long-term survival and function of pancreatic islets requires extensive experimental verification.