The Influence Of Different Hepatic Inflow Occlusion Methods On Liver Ischemia Reperfusion Injury

Objective To examine the influence of different hepatic inflow occlusion methods on liver ischemia reperfusion injury. Method Rats were under the portal venous clamp or the portal triad clamp. The serum ALT and AST of rats were measured and the observation of histological, ultra microstructure and TUNEL staining of liver tissue were taken. The expression level of c-fos and c-jun genes were measured by real time PCR. In clinical observation patients were assigned into three groups, the inflow occlusion methods of PTC , semi-hepatic inflow occlusion and portal venous clamp were applied to these three groups separately. Liver function after operation was recorded. Result Animal research revealed that PV occlusion can protect liver from I/R compare with PTC. C-jun expression with group A was lower than that of group B. This case research found that compared with PTC group, the portal venous clamp group has the same blood loss, but the liver function after operation was improved. Conclusion Portal venous clamping is superior than Pringle maneuver in relieve the I/R in liver. The mechanism of the effect probably has relationship with the downward of c-jun expression. The using of selective hepatic inflow occlusion in surgery can relief the liver injury after operation.