Study On Absorption Mechanism Of Cepharanthine

The purpose of this study is to investigate the absorption kinetics of Cepharanthine (CEP) , it is to modernize the theoretical basis of dosage forms selected and the method for screening prescriptions, optimizing techniques, evaluating quality. In our research we use three different models (in situ perfusion model in rats; ansa intestinalis model; kinetic research in vivo) to investigate the absorption kinetics of CEP. There are four parts in our research: in sute perfusion model in rats, ansa intestinalis model, kinetic research in vivo, evaluation the correlation among models.The method of in situ perfusion in ratswas adopted, and the HPLC methods were established. In this study, we investigated the absorption activities of CEP in the gastrointestinal tract. The results are as follows: in the gaster, the P%/h are: 5.13%, 5.99%, 6.01%; in the intestinal tract, the P%/h are: 18.89%, 15.10%, 14.32%.Then, the method of ansa intestinalis model in rats was adopted, and the HPLC methods for detection the CEP in rat's plasma were established. The results showed that: the absorption of CEP in rats is accord with two-compartment model. The result of in vivo models shows that: after i.v. and p.o. different doses of CEP, the physiological disposition of CEP in rats are both according with two-compartment model. The absolute bioavailability of CEP in rats is just 1.02%.Correlations between different models were evaluated by statistical test. There exist quantitative correlations between in situ model vs. in vivo. However the correlations aren't significant when the level of confidence interval is at 0.05 value. In summary, in situ perfusion model and in vivo model can be effective optional ways to investigate absorption mechanisms and anticipate absorption pattern. Utilizing two or more methods to synthetically evaluate the absorption results could ensure to obtain more reliable conclusions.Our research is to find the ways and evidences of enhancement bioavailability by investigating absorption mechanism and influential factor about CEP, and further integrate the development of CEP preparations from multi points and multi models based on the ideas of biopharmaceutics. The accomplishment of the project is a great achieve of modernization of CEP as well as of biopharmaceutics and pharmacokinetics and provide a novel idea and method for researching and developing modern preparations of CEP.