Study Of Aloe Vera Gel In The Treatmeat Of The Skin Lesion Caused By Laser

Background:It is a problem to be resolved to treat the woundcaused by laser, while aloe, as a chinses tradition medicineapplied for over one thousand years, shows marvelous effect onhelping the wound heal. The leaf of the aloe is in the conditionof natural gel, comparing with other oiments, gel has the advantageof good vebtilating, cleaning, moisturizing and so on. Atpresent, there are few reports about the systemic study of aloe gelin the treatment of skin lesion.Purposes:To create the animal model of skin lesion caused bylaser;to study the effect and mechanism of aloe gel in treatmentof skin lesion caused by laser.Methods: 56 japanese rabbits were chosen, half male and female, andthe weight of each was 2-2.5kg. After moule and anaesthesia, therabbits were treated with MedliteC~6-Nd:YAG laser(ConBio Company, USA), wave length:1064nm, spot diameter:3mm, energy density: 12J/cm~2, the distance between the light and skin: 8cm, irradiationtime: 40 seconds, frequency: 10Hz. The depth of the wound causedby the laser on the backs of rabbits was to the derm. The rabbitswere individed into 7 groups, 8 rabbits each group. Group 1 wasthe model control; Group 2 was positive controlⅠ(Erythromycinointment); Group 3 was positive controlⅡ(Fibroblast growthfactor, FGF), ;Group 4, 5, 6 were low-conCentration, middle-concentration and high-concentration aloe vera respectively; Group 7was blank control (no treatment by laser). The medicine and thealoe gel were applied on the lesion, once a day. The biopsy of thelesion was taken for HE staining on the day 3, 6, 9, 12 separately, in the meantime, we also mensurated IL-6, TNF-α, succinicacid dehydrogenase (SDH), hydroxyproline (HYP) respectively. Thedecrustation time and the heal time of the wounds were recorded.Results:①The decrustation times of the three aloe groups andthe positive control groups were shorter than the model controlgroup (p＜0.01);there was no statistic difference between the aloegroups and the positive control groups (P＞0.05).②The heal timesof the three aloe groups and the positive control groups were shorter than the model control group (p＜0.01);there was no statisticdifference between low concentration aloe group and the erythromycin control group and (P＞0.05); there is no statistic difference between the middle and high concentration aloe groups and theFGF control group (P＞0.05).③The IL-6 concentrations (ng/ml)of the aloe groups were lower than the positive groups and model group (p＜0.05); there were no statistic difference among thethree aloe groups (P＞0.05).④There was no statistic differenceof TNF-αconcentration(IOD) between the low concentration aloegroup and the erythromycin control group (P＞0.05), and theirTNF-αconcentrations were higher than the the model control groupand the blank group (p＜0.01), but lower than the middle and highconcentration aloe groups and the FGF control group (p＜0.05).⑤There was no statistic difference of SDH concentration (U/mgprot)between the low and middle concentration aloe groups and erythromycin control group (P＞0.05), and they all were higher thanthe model control group (p＜0.01), but lower than the high concentration aloe group and the FGF control group (p＜0.05); therewas no statistic difference among the high concentration aloegroup, the FGF control group and the blank group (P＞0.05).⑥The HYP concentration (μg/mg) of the aloe groups and positivegroups were higher than the model control group(p＜0.05);there wasno statistic difference of HYP concentration between the low andmiddle concentration aloe groups and the erythromycin control group (P＞0.05); the low concentration aloe group and the eryth romycin control group were lower than the high concentration aloegroup, the FGF control group, and the blank group(p＜0.05); therewas no statistic difference among the high concentration test group, the FGF control group, and the blank group (P＞0.05).Conclusions: Aloe can enchanced the synthesis and release ofTNF-α, downregulate the secrete of IL-6, moreover relieve the inflammation of the local lesion;promoted the activity of the SDHin the mitochondrion, increase the synthesis of HYP, so as tocontribute to the heal of the skin lesion. Furthermore, the higherthe concentration of the aloe gel was, the more obvious its effectwas. Compared with the positive groups, , the effect of thehigh-concentration aloe was better than the erythromycin and wasclose to the FGF as well.