The Expression And Significance Of Wnt-1 Induced Secreted Protein-1(WISP-1) In Human Rectal Tumor Tissue

Objective To explore the clinicopathological characteristics of Wnt-1 induced secreted protein-1 (WISP-1) in rectal tumor by analyzing the expression of WISP-1.Methods Applying RT-FQ-PCR, we quantified WISP-1 mRNA in 86 rectal cancers and 32 rectal adenoma issues from excised primary rectal Lesions. In each case, accompanying normal mucosa was collected for comparison. We determined WISP-1 protein expression by immunohistochemical LSAB technique and analyzed the PCR results by a new soft tool named REST^?Results 1. Among the 86 rectal tumor mRNA samples tested, WISP-1 mRNA overexpression was found in 65% (56 of 86) primary rectal cancers, ranging from 2 to over 30 times the level in normal matched rectal tissue (p = 0.001). WISP-1 mRNA expression level was correlated with Dukes stage, histclogical differentiation grade and lymph node status.2. Among the 32 rectal adenoma mRNA samples tested, WISP-1 mRNA overexpression was found in 37.5% (12 of 32) primary rectal adenoma (P = 0.042). WISP-1 mRNA expression level was correlated with size, histological shape, degree of atypical hyperplasia in rectal adenoma (P＜0.05).3.The WISP-1 protein expression was in accordance with mRNA expression level. From rectal adenoma to cancer, the expression of WISP-1 was gradually enhanced.Conclusions The features of WISP-1 expression from normal mucosa, adenoma and carcinoma of rectum suggests that WISP-1 gene is associated with the development of rectal tumor.