| Objective: To explore the method for isolating and culturing rat marrow-drived MSCs ,and observe the effects of MSCs transplantation on the locomotion recovery of complete-transected spinal cord injury in rats.Methods: .Under sterile conditions ,bone marrow cells from the tibias and femurs of the rats were collected as primary culture. The expression of CD54 and CD90 when subculture were identificated by immunocytochemistry. Thirty two adult Sprague-Dawley rats were randomly divided into transplantation group (n=16) and control grop (n=16) ,and spinal cord complete-transection was perfomed in all rats. MSCs of rats were transplanted into the injuryed spinal cord in transplantantion group immediately (Two rats of this group were injected MSCs which were prelabeled with BrdU),while control group was treated with injection of phosphate buffer saline (PBS). Then ,the rats′behaviors were assessed by BBB score 1-8w after transplantation respectively. In one week after operation, immunohistochemistry identification of BrdU to show whether MSCs survive or not after transplantation. Gross observation and histological examination were carried out on the injuried spinal cord four weeks after transplantation.Results: Immunocytochemistry showed that MSCs express CD54 and CD90. Treated rats generally showed better functional recovery than control rats after operation. BrdU positive cells could be found in the spinal cord injury site in one week after transplantation. Nerve fiber could be found in the spinal cord injury site in four weeks after transplantation.Conclusion: MSCs may survive in the spinal cord injury site by local injection immediately after spinal cord injury. MSCs may differentiate into neurons to promote recovery of conduction function in the injuryed rats spinal cord, and may contribute to regeneration of the injuryed axons.
|
PDF Full Text Request