Preparation Of RhGH-loaded Microspheres And Sustained Released Study In Vitro And In Vivo

Objective To prepare PLGA microspheres containing recombinant human growth hormone and study these sustained release in vitro and in vivo. Methods HSA microspheres were prepared by a double-emulsification solvent evaporation method. To design orthogonal experiment to optimize the preparation condition of rhGH-loaded microspheres and study characters of optimal rhGH-loaded microspheres. Results The diameters of optimal rhGH-loaded microspheres were mainly between 10μm and 40μm. The encapsulation efficiency and drug loading were (85.25±2.5)% and (11.52±0.42)% respectively.It showed good lente liberantes effect in vitro and in vivo. Conclusion This technique is simple and has a good reproducibility. It is a fine sustained dosage form for recombinant human growth hormone.