The Research Of Antineoplastic Effect Of PTX-PLGA Release Particles In Vitro

Objective1To prepare lactic-acid/glycolic-acid (PLGA) carrying paclitaxel(PTX) sustained-release polymer particles.2Detected the basic physical properties, release characteristics in vitro, also ultrasound imaging effect both in vivo and in vitro of the polymer particles.Methods1The sustained-release polymer particles ultrasound contrast agents carrying PTX were prepared with PLGA that could be biodegradable by double emulsion evaporation method and freeze drying techniques.2Morphological observation and analysis of particle size and drug distribution were detected used inverted optical microscope and scanning electron microscopy, Release characteristics of the polymer particles in vitro was detected by HPLC, observed ultrasound imaging effect in vivo.Results1Preparation of the PTX-PLGA contrast agent successful and got a high encapsulation efficiency also drug loading efficiency.2The PTX-PLGA sustained-release particles with the rules of spherical, uniform size and good dispersion under the light microscope, while had the round shape, smooth surface under the electron microscope. The average size of PTX-PLGA particles was (2.0±0.19)μm, average encapsulation efficiency was81.10%and average drug loading efficiency was5.68%. In vitro experiment showed that in the initial24h, PTX released by sustained-release particles was about5.78%, PTX was about60.83%30days later. PTX-PLGA particles also have good enhancement effects both in vitro and in vivo.ConclusionsSustained-release effect of the polymer particles ultrasound contrast agents prepared with double emulsion evaporation method and freeze drying techniques was obvious. The particles also with better ultrasound imaging in vivo, low toxicity and conform with the basic requirements of an ideal drug carrier. ObjectiveObserved the effect of antitumor and treatment of cells of human ovarine carcinoma cell line SKOV3used PTX-PLGA particles sustained-release in vitro.MethodsCultured cells of human ovarine carcinoma cell line SKOV3were treated with①RPMI1640as blank control,②normal saline(NS),③PLGA,④PTX and⑤PTX-PLGA microspheres. The proliferation of SKOV3cells were determined by MTT assay and the morphologic change observed under inverted microscope.ResultsMTT assay and the morphologic change showed that cells of human ovarine carcinoam cell line SKOV3in blank control, normal saline and PLGA group proliferate quickly and the antineoplastic rate of that have no statistical significance. In PTX and PTX-PLGA group,24h after treatment, the antitumor rate of PTX group is higher than PTX-PLGA(P<0.01); after48h and72h, the rate of PTX-PLGA is higher than PTX(P<0.01). And cell proliferation is stifled, extending over time, inhibitory effects of enhanced. ConclusionsPTX-PLGA sustained-release particles prepared by this method are stable and can obviously inhibit the proliferation of SKOV3cells in vitro.