Detection And Significance Of Helicobacter Pylori L Type And Mitochondrial DNA Microsatellite Instability In Esophageal Squamous Cell Carcinoma

Objective To detect the status of Helicobacter pylori L type(Hp-L) infection and mitochondrial DNA microsatellite instability(mtMSI) in esophageal squamous cell carcinoma(ESCC) and investigate the role of Hp-L and mtMSI and relationshipe between Hp-L,mtMSI and ESCC.Methods Hp-L infection rate was evaluated in 46 cases of ESCC , and compared to the normal tissues at the incised edge by three techniques such as Giemsa staining, immunohistochemistry(IHC) stain and polymerase chain reaction(PCR). The PCR technique was used to amplified the urase gene fragment of Hp. Hp-L morphological character was observed by Giemsa staining and light microscope.Then relationship between Hp-L infenction and ESCC was analyzed.The mitochondrial DNA(mtDNA) was amplified by genepha long PCR kit. PCR product was regard as template.(C)n and (CA)n locus exist in the D-loop zone of template mtDNA were amplified.The PCR product′s MSI was analyzed by PCR-SSCP technique to investigate the relationship between mtMSI and ESCC or Hp-L.Results 1.The Hp infection rate was 65.2%(30/46) in ESCC with significant difference compared to normal control tissues. The main shape of Hp was Hp-L type which distribute into or beside the cancer nest.There lies correlation between Hp-L infection and ESCC with significant relationships between Hp-L infection with the invasive depth of the tumor tissues and the regional lymph node metastasis and no significant relationship with other clinicopathologic charecteristics such as the sexulity, the cell differentiation, the tumor size and so on. 2. mtMSI detection rate was 32.6%(15/46) in 46 case of ESCC. It was incidence in 13 cases of ESCC that mtMSI was examined at (C)n locus of D-loop zone, and in 5 cases at (CA)n locus, and in 3 cases both at (C)n and (CA)n. mtMSI were indiscoved in nomal tissues at the inside edge. There was no significant relationship between mtMSI and clinicopathologic charecteristics. 3. The mtMSI incidence in the 30 cases of ESCC which infected Hp-L was 46.7%(14/30). However, the mtMSI incidence in the 16 cases of ESCC which was not infected Hp-L was 6.25%(1/16). The difference of the mtMSI detection rate has statistical significance between the ESCC infected Hp-L and that disinfected Hp-L.Conclusion 1.Hp, espacilly Hp-L infection, is frequent phenomenon in ESCC. 2.Hp-L play an important role with tumorigenesis and development of ESCC ,and has a relationship with tumor invasion and metastasis of ESCC. 3. MSI of mitochondrial D-loop zone (C)n locus play an important role with tumorigenesis and development of ESCC, and has relationship with Hp-L infection. 4. The association detection of the Hp-L and the mtMSI is possibly helpful for the diagnosis of ESCC.