CAGA - Positive Helicobacter Pylori Culture Filtrate On Proliferation Of Esophageal Carcinoma Cell Line Ec-109

Esophageal carcinoma is one of the common malignant tumors, which results in 300,000 deaths every year worldwild. In china, it also severely threatens people's lives. For decades, to explore the etiology and pathogenesis of esophageal carcinoma, researchers have done a lot of epidemiological survey, population census, experimental research in its high spot, but so far the exact etiology and pathogenesis are not very clear yet. Preliminary results indicate that esophageal carcinoma is a multi-factor, multi-stage and progressive evolutional process, it may connect with geographical environment, diet, microbial infection, smoking, alcohol, genetic factors and so on.In recent years, with the in-depth research of biological causes, more and more attentions have been paid to the relationship between Helicobacter pylori(H. pylori) and esophageal carcinoma. H.pylori is a gram-negative, micro-aerobic pathogenic bacteria which colonizes human gastric mucosa. It was first isolated from chronic active gastritis gastric mucosal biopsies by Marshall and Warren in 1983. The discovery of H. pylori has greatly promoted the developpment of digestive disease terminology. Over twenty years, through a great deal of epidemiological and experimental research, it has been proven that H. pylori is closely related to human chronic gastritis and peptic ulcer, while it is risk factor induced gastric cancer and mucosa-associated lymphoid tissue (MALT) as well, in addition, it was announcad theâ… type cancerigenic factor in 1994 by WTO. Though scholars both domestic and overseas have noticed that H. pylori infection rate of patients with esophageal carconoma is higher than that of the normal people and H. pylori infection has been detected from esophageal cancer tissues, the exact relationship between H. pylori and esophageal carcinoma is uncertain and the mechanism is not unclear. Among the diverse virulence factors of H. pylori, distinctive pathogenic factors CagA, VacA are under the spotlight. In our research, in order to investigate the significance of H. pylori infection in the occurence and developpment of esophageal carcinoma, slices of esophageal cancer samples were Giemsa dyed, esophageal carcinoma cell line(EC-109) was cocultured with H. pylori broth culture filtrates, which was followed by cellular morphological observation, detections of cellular proliferation with MTT method, and cell cycle with flow cytometry.The results indicate that Helicobacior infection exists in esophageal carcinoma specimens, after EC-109 was cocultured with H. pylori CagA+ broth culture filtrates of different concentration, the cells appear obvious vacuole in a concentration-dependent manner. Cell proliferation was obviously observed at lower concentration of the culture filtrates, but cell apoptosis appeared at higher concentration.Results:CagA+ H. pylori can induce the imbalance between cell proliferation and apoptosis of EC-109 cell line, which suggests that chronic infection induced by lower degree reproduction of H. pylori might be one of the causes of contributing to generation and devlopment of human esophageal carcinoma.