Gut Barrier Change And Acute Lung Injury After Rat Intestinal Ischemia-Reperfusion May Be Modulated By Ligation Of The Mesenteric Lymphatic Duct

Objective. To investigated the changes of gut barrier injury, acute lung injury,and system inflammatory response to the ligation of the mesenteric lymphatic ductbefore intestinal ischemia and reperfusion. To value the potential role of mesentericlymph in the development of SIRS and MODS.Methods. Rats were divided into four groups: group 1, blank; group 2, simplelaparotomy (sham); group 3, intestinal ischemia and reperfusion (Ⅰ/R); and group 4,intestinal ischemia and reperfusion with the mesenteric lymphatic ductligation(Ⅰ/R+L). Gut barrier injury, the bacterial translocation and the level ofendotoxin, D-lactate and cytokines in systemic circulation were measured.Results. The mucosa thickness and villus height of jejunum and ileum afterⅠ/Rare significantly decreased compared with blank and sham group (P＜0.05). But thereis no significantly change between theⅠ/R andⅠ/R+L group. There is no bacteria beendetected in all groups in the blood from the portal vein. There is no bacteria beendetected in blank and sham group, but it's 40％inⅠ/R, and 20％inⅠ/R+L group(n=10). The level of endotoxin, D-lactate, cytokines, such as IL-1β, IL-6 and TNF-α,and ICAM-1 are significantly increased afterⅡ/R. The increase of endotoxin andD-lactate ofⅠ/R are significantly decreased by mesenteric lymphatic duct ligation (P＜0.05).Conclusion. IntestinalⅠ/R could obviously change the morphology of jejunumand ileum. The ligation of the mesenteric lymphatic duct before intestinal ischemiaand reperfusion was found have no protection effect to the gut morphology, but itcould reduce the bacterial translocation and the level of endotoxin and D-lactate insystem circulation. The duct ligation was found to reduce the degree of subsequentlung injury and PMN recruitment.