The Experimental Study For The Protective Effect Of Glutamine On Intestinal Mucosa During Hepatic Ischemia/Reperfusion Injury In Rats

Objective To investigate the protective effect of glutamine on intestinal mucosa during hepatic ischemia/reperfusion injury in rats and discuss its possible mechanism.Method 18 male Wistar rats were randomly divided into glutamine precondition group (G group) and 0. 9% Nacl solution control group(C group). All rats were cannulated in right external jugular veins; and infused 0. 9% Nacl solution through mini-pumps for 1 day, then respectively changed into continuous infusion for Dipeptiven solution and 0. 9% Nacl solution for 3 days. The liver was subjected to warm ischemia by cross-clamping the hepatoduodenal ligament for 40 minutes according to Pringle' s maneuver, then declamping to restore the blood flow. We got the mesentery lymphoid nodes in ileocecal part sterilely for germiculture after 1 hour of reperfusion, examining the bacterial transloction;then cut partial ileac tissues, which were treated routinely for later mucosal morphological observation under light microscope and scanning electron microscope, estimating mucosal injury. Results 1. Bacterial translocation:After ischemia for 40 minutes and reperfusion for 1 hour, the bacterial content of mesentery lymphoid nodes in G group (492.27 247.52 CFU/g) was obviously lower than that in C group (3865.02 1681.07 CFU/g) , both were different significantly(P<0.05). In this study, we chose qualitatively partial bacterioflora and bacteria inside guts for identifying bacterial type, indicating that both were homologous. 2. Morphological changes:After ischemia for 40 minutes andreperfusion for 1 hour, the gut injury in G group was mild than in C group.Conclusion precondition with glutamine intravenously before operation has protective effect on intestinal mucosa during hepatic ischemia/reperfusion injury in rats. The protective effect may be mediated by promoting proliferation and restoration of mucosal cells,inhibiting the increased permeability of mucosal barrier, influencing the formation of bacterial group in guts, inhibiting bacterial group unbalance and maintaining the immune function of GALT, as well as the GSH content in liver.