| Objective To explore the mechanism and effect of allogenic BMSCstransplantation on neovascularization in rat model of hindlimb ischemia.Methods BMSCs in rats were separated from bone marrow with densitygradient centrifugation and wall sticking screening, which were amplifiedand induced in vitro. The induced cells were evaluated by the cell morphology,immunotochemistry staining and fluorescene activated cell sorter (FACS)analysis. After ischemia models were induced by surgical ligtion and cuttingoff right femoral artery as well as all of their main branches in each rat,which were divided into transplantation group(n=10) and control group(n=10)randomly. The BMSCs of passage 4 were labeled with bromodeoxyurdine (BrdU).BMSCs were directly injected into the ischemic hindlimb muscules intransplantation group. The functional assessment of the ischemic hindlimbwas performed after ligation. Four weeks later, the micro-vessel density(MVD)were observed and evaluated by means of DSA and immunohistochemistry beforethe rats were sacrificed. Expression of CD34mRNA and VWFmRNA of ischemic musclewere determined by reverse transcription-polymerase chain reaction (RT-PCR). Thelevels of VEGF, bFGF in the ischemic musles were measured by ELISA.Results Allogenic BMSCs could survive in the ischemic hindlimb. Thefunctional assessment of transplantation group was lower than that ofcontrol group (P＜0. 05～0.01). The MVD, expression of CD34mRNA and VWFmRNAof ischemic muscles in the transplantation group were significantly higherthan that in the control group (P＜0.005, P＜0.01, respectively). The levelsof VEGF, bFGF in the transplantationgroup were also significantly higher than thatin the control group at the 4th weeks after treatment (P＜0.05～0.01). Conclusion The implantation of allogenic BMSCs could induceneovascularization in the rat ischemic hindlimb, and VEGF, bFGF secreted byBMSCs might play a role in the neovascularization.
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