Study Of Zinc, Selenium On Arsenic Induced Lipid Peroxidation And The Ability Of Arsenic To Induce Metallothionein In Mice

Objective: To investigate the biological effects of the different concentrations of sodium arsenite (NaAsO₂) in mice, and to explore zinc, selenium on arsenic induced lipid peroxidation and metallothionein synthesis in mice. Methods: Superoxid(SOD), glutathione peroxidase (GSH-Px),malondialdehyde (MDA) and glutathione S-transferase(GST) in serum and organization were measured and analyzed by enzyme linked immuno-absorbent assay, metallothionein protein were determined by graphite furnace atomic absorption spectrometry.Interleukin-l(IL-l), Interleukin-6(IL-6) in serum of mice were measured by enzyme linked immuno-absorbent assay. Results: (1)Compared with the control group, the MDA contents were higher, while the GSH-Px and SOD levers were lower in low, middle and high arsenic groups. On the contrary, the MDA contents decreased and the GSH-Px and SOD levers were higher in Zn and Se groups. Compared with high arsenic group, the MDA contents were lower and the GSH-Px and SOD levers were higher. (2)Compared with the control group, GST contents in serum were higher in low, middle and high arsenic groups, liver GST contents were higher in middle,high arsenic and H+Zn,H+Se groups, the GST contents in serum and liver were lower in Zn and Se group, the contents of GST were higher in those groups except for the middle arsenic than that of the high arsenic group (P<0.05). (3) Compared with the control group and high arsenic group,the MT contents of liver and brain were higher in low arsenic, Zn and Se groups, while liver MT contents had statistically significant results (P<0.05), but brain MT was no statistical significance (P>0. 05). (4) IL-1 contents in low and middle groups were higher than those in control group in serum of mice (P<0. 05), IL-6 contents were higher in low arsenic group than those in control group (P<0. 05). Compared with the high arsenic group, the IL-1 and IL-6 contents were increased in those groups except for the middle arsenic group (P<0. 01). (5) MT has positively correlation to SOD,GSH-Px,IL-1 and IL-6. MT has negatively correlation to MDA and GST. Conclusion: Arsenic can induce lipid peroxidation and metallothionein protein. Zinc, selenium can against arsenic toxicity and reduce oxidative damage. Metallothionein synthesis may influence the risk of arsenism. Metallothionein can be considered as endemic arsenic susceptibility markers.