| Along with the medicinal plant ectomycorrhizal fungi in-depth studies, medicinal plants found within VAM fungi secondary metabolites with host plants containing the same or similar to the active ingredients. Moreover, the production of medicinal plants is often seasonal, pests and other natural conditions adversely affected and therefore, Medicinal plant resources to fend reasonable alternatives have become today's medicinal plant research one of the hot issues. The experiment of medicinal plants within VAM fungi, plants for medicinal become reasonable alternatives for the possibility.Experimental use of the separation of the organization, from 16 kinds of medicinal plants isolated from the roots of Health within 11 mycorrhizal fungi. Study on biological characteristics, antimicrobial activity, effective chemical composition and molecular biology of the five strains.There are five strains be tested. They are ectomycorrhizal fungus isolated from Platycodon grandiflorum (JG1102), Saposhnikovia divaricata (FF0121), Sophra flavesces (KS1118 and KS1220), Polygonatum odoratum (YZ1104), Scutellaria baicalesis (HQin0116). There are 4 species could be identified by the morphological characteristics—JG1102 (Alternaria platycodonis), FF0121 (Alternaria radicina), KS1220 (Alternaria azukiea), and YZ1104 (Fusarium avenaceum)Screening appropriated LD1025, RS1109, JG1102, DS0216, KS1118, KS1220, XYS0305 and YZ1104 growth of carbon and nitrogen sources from five different carbon and nitrogen sources. Comprehensive results, and cost savings of many factors, including raw materials, We were chosen as glucose and potatoes within ectomycorrhizal fungi to develop the best source of carbon and nitrogen, in the tested within VAM fungi culture, Potato use glucose (PDA) medium can achieve the best results. The optimal growth temperature of the study, the experiments of 20～25℃for ectomycorrhizal fungi within the optimum temperature.Screening 3 strains in the 4 species, which have antibacterial activities. There are JG1102, YZ1104 and HQin0116. The antibacterial results showed that: the concentrated ferment liquid, methanol crude extract and ethanol crude extract all had presented antibacterial activities to all the tested bacteria. Moreover, the antibacterial rate of methanol crude extract almost higher than ethanol crude extract. But the extracts from water have no antibacterial activities to all the tested bacteria. Used thin-layer chromatography, outspread dose were CHC13: MeOH (8.5: 1.5) and CHC13: MeOH (7:1) (with a little ammonia and diethylamine), color reagent was 10% oil of vitriol. Observe the fluorescent spots and color sports. The results showed that: the secondary metabolites of JG1102, YZ1104 and HQin0116 have the same or similar alkaloid and flavone compositions as the plants they parasitized.Used ITS1 and ITS4 to be primers, PCR amplification the rDNA that extract from KS1220, sequencing the amplification products later. The result showed that: this endocorrhiza has highly homology with the soil fungus in genebank.To sum up, the achievements of this research are as follows:It's the first time to use tissue separation and identification of morphological method to determine Alternaria platycodonis as endocorrhiza for Platycodon grandiflorum, Alternaria radicina as endocorrhiza for Saposhnikovia divaricata, Alternaria azukiea as endocorrhiza for Sophra flavesces, and Fusarium avenaceum as endocorrhiza for Polygonatum odoratum.It firstly to screening 3 strain that have antibacterial activities. There are JG1102 (Alternaria platycodonis),YZ1104 (Fusarium avenaceum) and HQin0116 (endocorrhizas isolated from Scutellaria baicalesis). And it proved that they have the same or similar alkaloid and flavone compositions as the plants they parasitized. |
