An Experimental Study Of Allograft Decalcified Bone On Repair Of Bone Defects

prefaceAs the scaffold of human body, skeleton function as support and weight-bearingsystem, hematogenesis. Owing to trauma,tumor and infection, bone defects are quitecommon clinically. Although satisfactory result had been received in the treatment of bonedefect by the way of autologous bone, lots of complication and additional injuries alsoappeared. Due to the limit source of autologous bone, allograft bone has been adoptedwidely in clinical experience. In some cases, as a result of antigenity of allograft bone andhost rejection, failures are inevitable.With the development of research of bone tissue engineering, research focus ofscaffold material of tissue engineering has been transferred to seek biological materialwhich is of structural charactristicalike human bone tissue. Two thoughtson materialmanufacture was put forward: 1, ceramics, degradable polymer material and its bio-derivedmarerial; 2, natural material and its bio-derived material, such as allograft bone andautologous bone.ObjectiveTry to implant allograft bone which decalcified differently in the site of bonedefect, seek the quantity standard of decalcified degree through observation of repair resultanf provide relevant property data of scaffold material of bone engineering viacomparative research on bio-derived material.Methods20 new Zealand white rabbits were chosen and implanted with manufactured PDCBon the site of their 10mm radius defect respectively. There were individed into four groups and collateral control was adopted. Every 10 rabbits were sacrificed in 30,60dayspostoperatively, immunohistochemically, biomechanical property, components andmorphological feature of manufactured PDCB were assessed simultaneously.ResultsThe result of 30,60days after operation showed that healing velocity of allograftbone decalcified to certain degree was quicker than that of common allograftbone, decalcified allograft bone had the excellent feature inhistocompatibility, three-dimensional structure,plasticity and mechnical.Conclusions1. As an implant, 81.1% partially decalcified allograft bone is appropritiate for therepair of bone defects.2. Allograft bone, that had better biological characteristics and simple manufacturedprocedure, is a good scaffold for tissue engineering.