The Relation Between The Biology State And The Illness State Of Chronic Hepatitis B HBV

ObjectiveTo understand the HBV replication works by determining the relationship betweenHBV cccDNA and HBV DNA in peripheral blood mononuclear cells and liver tissue inchronic hepatitis patients. From the study, we had an objective estimation for HBVcccDNA and HBV DNA replications.MethodA group of blood mononuclear cells with chronic hepatitis B and a group of livertissues with chronic hepatitis B were examined. By Fluorescent Real Time QuantitativePCR method, the quantity of HBV cccDNA and HBV DNA can be determined andanalysed in blood mononuclear ceils and liver tissue samples.ResultsThe concentration of HBV cccDNA was 0～5.06×10~7copy / ml in liver tissue and0～6.37×10~5copy/ml in blood mononuclear cells. Positive rates of HBV cccDNAwere 93.7% and 84% in liver tissue and blood mononuclear cells respectively inchronic hepatitis patients. For the same patient, the concentration of cccDNA in livertissue was 10.7～9.2×10~5 times to that in blood mononuclear cells. The concentration ofHBV DNA of 0～1.2×10~9 in liver tissue and 0～1.56×10~7 in blood mononuclear cellswere detected. It was determined that the concentration of HBV DNAwas closelyrelated to HBV cccDNA in liver tissue(r=0.878, P＜0.01) and in blood mononuclearcells (r=0.897, P＜0.01). The concentration of HBV DNA in liver tissue was positiverelated to the concentration of HBV DNA(r=0.489, P＜0.01) and HBVcccDNA (r=0.460, P＜0.01)in blood mononuclear cells. The concentration of HBVcccDNA inliver tissue was positive related to the concentration of HBV(r=0.516, P＜0.01) andHBVcccDNA (r=0.473, P＜0.01)in blood mononuclear cells. There was no relationshipbetween HBV cccDNA in liver tissue and the rate of liver tissue inflammation werefound (P＞0.05). The alanine aminotransferase of liver function was positive related toliver tissue inflammation in hepatitis patients.Conclusionthe concentration of HBV cccDNA in liver tissue determined by Fluorescent RealTime Quantitative PCR is the most reliable factor to estimate the HBV replications.The results are significant for CHB diagnose and anti-HBV therapy. It was determinedthat the concentration of HBV DNA was closely related to HBVcccDNA in liver tissueand in blood mononuclear cells. The concentration of HBV DNAin liver tissue waspositive related to the concentration of HBV DNAand HBVcccDNA in bloodmononuclear cells.HBV DNA in serum was found that it had no relationship with HBVcccDNA and HBV DNA in liver tissues and in blood mononuclear cells. HBV DNA inliver tissues and blood mononuclear cells and HBV cccDNA in liver tissues and bloodmononuclear cells and serum had no relationship with liver tissue inflammation inhepatitis patients. The alanine aminotransferase of liver function was positive related toliver tissue inflammation in hepatitis patients.