| Periodontitis is a chornic infectional inflammation that emerges on the. periodontium and infected by dental plaque.It usually induces the disorganization of periodontal tissue, which will eventually develop into attachment loss with the defluxion and lossening of tooth, as is the main cause of losing tooth for adults. It is indicated by the research that plaque microbes is the initiation factor that causes periodontal disease as well as the destruction of periodontium, so that the control of the plaque is the key of succeeding in treating the periodontitis.Nearly 30 years of pharmacology study confirmed that traditional Chinese medicine cinnamon volatile oil major components of cinnamaldehyde inhibit ulcer have, analgesic, antipyretic and promote blood circulation, Anti-bacteria, fungi, molds, viruses, cancer, etc., and low toxicity. Which, Anti-Inflammatory the role of periodontal disease have a therapeutic significance.In cinnamaldehyde applications in the treatment of periodontal disease, cinnamaldehyde in the in vitro anti-periodontal bacteria and anti-inflammatory role of research, also need to be aware of cinnamaldehyde periodontal functional cells.To clear cinnamaldehyde periodontal main function of cells -- osteoblasts proliferation of osteoblast function and whether, In this experiment osteoblasts for experiments, periodontal tissue of the main functions cell——osteoblasts is for experiments, measured by MTT assay five concentration of drug group and the control group living cells OD (optical density, OD) indirectly calculated for different concentrations of bone cell growth inhibition rate of cinnamaldehyde, and Based on the determination of the activity of alkaline phosphatase, calcium content measured Alizarin red staining calcified nodules and other experimental methods for investigating the influence to osteoblast function of different concentrations of the drug group. The results are as follows:1. Low concentrations of cinnamaldehyde extract 8mg/L,16mg/L group compared with the control group.cells higher levels ofvalue-added,high concentrations as 128mg/L,64mg/L.The dilution ratio,the osteoblasts value is to some extent the decline,With the number of days increased cell growth is still the trend,the same concentration as low 16mg/L profenofos, and the control group,compared with obvious inhibition;2. Cinnamaldehyde extract the dilution ratio 8mg/L,16mg/L, with the control group, osteoblasts ALP activity and calcium content increased significantly (p＜0.05), while 128mg/L, 64mg/L,32mg/L the high concentration group with the control group, osteoblasts ALP activity and calcium content of various magnitudes lower the difference was significant (p＜0.01).3. 16mg/L,8mg/L diluted than in the role of liquid cinnamaldehyde, with the control group, osteoblast mineralization nodules for a small increase (p＜0.05), while the dilution ratio 128/L, 64mg/L,32mg/L of cinnamaldehyde extract role 21 days later, with the control group, reducing the number of mineralized nodules, the difference was significant (p＜0.05)In summary, low concentration(16mg/L 8mg/L) cinnamaldehyde on osteoblasts have some positive effect, a high concentration (128mg/L, Fas 32g) cinnamaldehyde extract of osteoblast cells with a certain effect, right osteoblasts value, differentiation, maturation and function of varying degrees of impact.This experiment in vitro cell culture technology, from the cell biology level of the Chinese cinnamon major components of cinnamaldehyde right mouse bone cells and alkaline phosphatase activity and the content of calcium, for the research and development of cinnamaldehyde in the treatment of periodontal disease with the application of theoretical and experimental evidence.
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