Relationship Between Peroxisome Proliferators Activated Receptor Gamma C161-T Gene Polymorphism And Coronary Atherosclerosis

Coronary Heart Disease is a multi-factors disease. Scientists and doctors are paying more attention to genetic factor as one of the important mechanism of CHD. Peroxisome proliferators activated receptor is an inter-nuclear hormone receptor, which can be actived by peroxisome proliferators and fatty acid. Peroxisome proliferators activated receptor gamma(PPARγ)is one pop-investigated type. Many investigations showed that PPARγshould attend the pathology process of atherosclerosis, but wether gene polymorphism of PPARγis associated with the occurrence and development of coronary atherosclerosis is not reported yet. Aim: The purpose of this study is to analyze if gene polymorphism of PPARγC161-T is an associated gene factor of the occurrence and development of coronary atherosclerosis through studing the association between gene polymorphism of PPARγC161-T and CHD and straitness degree of coronary artery.Part OneMethod: A case-case study was designed in this study. All the cases were divided into CHD group and control group by the result of Coronary Angiography.161cases were included in the CHD group,and 103 ones were included in control group. Blood pressure, Body mass index(BMI), waistline, fasting blood sugar(FBG), 2Hbg, high-density lipoprotein cholesterol(HDL-C), Low-density lipoprotein cholesterol (LDL-C), total cholesterol(TC), Triglyceride(TG) and gene polymorphism of PPARγC161-T were examined. The polymerase chain reaction-restriction fragment length polymophsis (PCR-RFLP) method were used to analyze the gene polymorphism of PPARγC161-T. The association between gene polymorphism of PPARγC161-T and CHD and the occurrence of coronary artery was studied.Result:①Waistline and 2hBG were higher in CHD group than in control group.②The freqency of CC type of PPARγwas higher in CHD group than in control group(P<0.05), while The freqency of CT type was lower in CHD group(P<0.01). There was no difference between the freqency of C(86.4%) and T(13.6%) in CHD group and the freqency of C(80.1%) and T(19.9%) in control group.③The gene polymorphism of PPARγC161-T was an independent risk factor which was associated with the occurrence of CHD after farraginous factors were excluded.Conclution: The gene polymorphism of PPARγC161-T was associated with the occurrence of CHD.Part TwoMethod: The polymerase chain reaction-restriction fragment length polymophsis (PCR-RFLP) method were used to analyze the gene polymorphism of PPARγC161-T. All the cases were divided into CC group (186 cases), CTgroup (71 cases) and TT(7 cases) group according to the result of the gene polymorphism of PPARγC161-T. The mass Blood pressure, Body mass index(BMI), waistline, fasting blood sugar(FBG), 2hBG, high-density lipoprotein cholesterol(HDL-C), Low-density lipoprotein cholesterol (LDL-C), total cholesterol(TC), Triglyceride(TG) were examined . All the cases accepted coronary angiography, and the Gensini score was calculated. The association between gene polymorphism of PPARγC161-T and strait branches and straitness degree of coronary artery were studied.Result:①The mass, BMI and 2hBG were higher in CC group than in CT group.②The frequence of three branches pathological changes was higher in CC group than in CT group(ⅹ~2=7.579,P=0.005),while the frequence of 0 brance change was lower in CC group than in CT group(ⅹ~2=6.752,P=0.01), which proved that persons with CC type were easy to suffer multi-branches pathological changes in coronary artery compared with those with CT type.③The gene polymorphism of PPAR γC161-T was concluded in multi-factors regression of Gensini score(P=0.041).Conclusion: The gene polymorphism of PPARγC161-T may be one of the independent factors associated with the straitness degree of coronary artery. The gene polymorphism of PPARγC161-T was one of the important genetic factors of the occurrence and development of CHD.