Effects Of Carbon Disulfide On Pathology, Apoptosis, Steroli Cell And Gene FasL Expression Of Testes In The Male Rats

Carbon disulfide (CS2) is a kind of popular organic solvent. Many epidemiological studies showed that CS2 could affect sexual function of male workers exposed. In the animal experiments, we can also discover that atrophy of testis of the male animal exposed CS2, decrease of sperm count and increase of aberration rate of spermatic chromosome. In our research, we observed pathological changes and apoptosis of testis in the male rats treated with CS2. And we also studied cytoactive and gene FasL expression induced by CS2 in steroli cell. The aim of this research is to explore the possible mechanisms of CS2 in structure and cells of the testis.1. Experiments in vivo40 male rats were randomly divided into four groups. It took ten weeks for the rats to breath CS2 in different concentrations (0, 50, 250, 1250mg/m3). After 10-week treatment, pathological changes and apoptosis of testis were observed with HE strain, transmission election microscope and TUNEL. The result of HE stain was that edema of interstitial tissue of testis aggravated as concentration of CS2 increased. The counts and layers of spermatogenic cells obviously decreased in 1250mg/m3 concentration group. Endoplasmic reticulum of Leydig cell extended and mitochondria of spermatogonium and primary spermatocytes swelled in 50mg/m3 and 250mg/m3 concentration groups. In 1250mg/m3 concentration group, there're many cavitates in the endochylema of Leydig cells, stertoli cells and spermatogenic cell. Mitochondria of spermatogoniums and primary spermatocytes became sweller. Mitochondria of spermatids also swelled and stucture of microtubule of sperms were broken. The result of TUNEL was that apoptosis index of the cells in the testis increased as the concentration of CS2 increased. Apoptosis index of 250mg/m3 and 1250mg/m3 concentration group was significantly higher than the control group (P<0.05). The abnormal apoptosis cells were spermatocyte.2. Experiments in vitroThe target cell is Sertoli cell (it is the only body cell in testicular seminiferous tubule). Steroli cells were exposed to different concentrations of CS2 (0, 60, 120, 240μmol/m) for 24 hours. Survive rate, apoptosis rate, and expression lever of gene FasL were measured using MTT, FCM, and RT-PCR methods respectively. Steroli cell survive rate decreased as the concentration of CS2 increased. The survive rate was significantly lower than the control group when the concentration of CS2≥240μmol/ml (P<0.05).Apoptosis rate increased as the CS2 concentration increased. Apoptosis rate was significantly higher when the concentration of CS2≥240μmol/ml(P<0.01). Expression lever of the FasL significantly increased in the concentrations group of CS2 (P<0.01).We can see from the above experiments: CS2 lead to edema of interstitial tissue. The counts and layers of spermatogenic cells decreased. Ultrastrycture of endoplasmic reticulum of Leydig cells and microtubule of sperm were broken. CS2 is cyotoxic to steroli cells. It could cause apotosis of steroli cells. Expression of the FasL significantly increased. The abnormal expression of FasL is the possible mechanisms of CS2 in male reproduction.