Dynamic Expression Of VEGF And PEDF In Experimental Rat Corneal Neovascularization Development

【Objective】To observe the dynamic expression of vascular endothelial growth factor (VEGF) and pigment epithelium-derived factor (PEDF) in alkali burn–induced experimental rat corneal neovascularization (CNV) tissues in the course of CNV development,and discuss the correlation between the fluctuating of the expression of VEGF/PEDF and the course of CNV development.【Methods】Corneal neovascularization model was induced by alkali burn on 30 adult male Sprague-Dawley rats(30 right eyes),who were divided randomly into 6 groups according to different time point after burn. Another 5 rats without corneal burn was 0 day group. Observed CNV development with Digital slit lamp image system on the 1st,3rd,5th,7th,10th and 14th day after burn respectively,and measured immunohistochemical reaction of VEGF and PEDF by immunohistochemistry. The results of VEGF and PEDF immunohistochemical reaction were analyzed by picture quantitative analysis techniques and described with average optic density(AOD).【Results】In CNV tissues, the positive immunologic reaction of VEGF is most apparent during the 3rd -7th day and very weak after 14th day while of PEDF is most apparent during the 7th-10th day and then declines remarkably at the 14th day. There's certain time-correlation between the dynamic expression of VEGF and PEDF and the development of CNV. 【Conclusion】The expression of VEGF and PEDF is remarkably detected in experimental rat CNV tissues, and the fluctuate of which is consistent with the development of CNV. The breakdown of the balance between them may play a role in CNV occurrence and development.