Screening Of The Alkaloids Of Berberis Anhweiensis Ahrendt And Determination Of The Thiosulfinates Content In Allium Cepa L

Berberis anhweiensis Ahrendt is one of the Chinese medicine material gathering drug, which could cure dysentery, gastroenteritis, pharyngolaryngitis, conjunctivitis and so on. Allium cepa L is honored as the empress of vegetables in Europe gathering drug and edible.This paper studies the alkaloids in Berberis anhweiensis Ahrendt, the screening of bioactive constituents from Berberis anhweiensis Ahrendt and the determination of total thiosulfinaes in fresh onion and onion extract from different producing area. The results are as following in this paper:1. Six protoberberine alkaloids were isolated and identified as berberine, palmatine, dehydrocapaurinine, jatrorrhizine, columbamine, jatrorubine by silica column chromato--graphy and spectral analysis. The six protoberberine alkaloids were at the first time isolation from Berberis anhweiensis Ahrendt. The palmatine content in this plant is higher than in other Berberis plants.2. To apply conidia of Pyricularia Oryzae for the screening of bioactive constituents from Berberis anhweiensis Ahrendt. By detecting deformation of mycelia germinated from conidia of P.Oryzae P-2b, three bioactive compounds (I , II, VI),the MMDC(minimum morphological deformation concentration) of which is 12.5,50 and 100/μg·mL^-1 respectively were screened from this plant. This bioassay method was efficiently applied to the primary screening of bioactive compounds from Berberis anhweiensis Ahrendt.for the first time.3. Thiosulfinafes are the best known biological active compound of onion. It has described a simple spectrophotometric assay for the determination of total thiosulfinaes in fresh onion and onion extract from different producing area using cysteine and DTNB. In two extraction methods, there exist significant difference among the content of the total thiosulfinaes in onions from Shanghai, Nanjing; and in different origin, there exist significant difference between the content of the total thiosulfinaes in the fresh juice and the chloroform extract.In fresh onion and the dry matter of onion, the content distribution is the same, which the content of the total thiosulfinaes is higher in fresh onion juice of Tai'an.