Determination Of Trans-and Cis-resveratrol And Piceid From Polygonum Cuspidatum And Vitis Amurensis By HPLC-ESI-MS

Resveratrol (3,4',5-trihydroxystilbene) is a naturally occurring stilbene derivative, which exhibits extensive physiological and pharmacological effects, including antioxidative, free radical scavengering, anti-inflammatory, anti-tumor, phytoestrogen-like, immunoregulation, antiplatelet, regulating blood lipid, preventing artherosclerosis, and hepatoprotective effects. It is a very promising natural anticancer agents and health product. Resveratrol is widely distributed in the genus of Polygonum (Polygonaceae), Vitis (Vitaceae), Arachis (Pulse), Eucalyptus (Myrtaceae), Chenopodium (Liliaceae), and Artocarpus (Moraceae). The content of resveratrol in giant knotweed rhizome (Polygonum cuspidatum) and Vitis is the highest.There are trans- and cis-resveratrol and their glycosides piceid (3-O-β-D-glucosides) in nature, while the trans-configuration is more stable. The existing forms of resveratrol and their contents are different in different plants, and the content of resveratrol in the same plant species also varies due to different growing places and different collecting seasons. In order to find the natural medicinal material with high resveratrol contents, we assayed the contents of different forms of resveratrol in P. cuspidatum rhizome collected from different regions, and also assayed the content of resveratrol in different parts of Vitis amurensis Rupr. collected in different seasons.After a comparison of the methods reported in the literatures, the ultrasonic extraction technique with high efficiency and simple operation was adopted to extract resveratrol from P. cuspidatum rhizome. For the first time, we determined the content of resveratrol and its glycoside piceid (3-O-β-D-glucosides) in P. cuspidatum rhizome collected from eleven regions in China by liquid chromatography-mass Spectrometry. The results showed that there was also small amounts of cis-resveratrol in P. cuspidatum rhizome besides trans-resveratrol and trans-resveratrol-glucoside (3-O-β-D-glucoside), which provides the basis for deciding standard components of active ingredients of P. cuspidatum rhizome and its quality control standards.In order to inspect the variation of the content of resveratrol in different parts of Vitis amurensis in different seasons, orthogonal design was used to search the best extraction method for the first time. We gathered roots and stems from January to December, leaves from April to October, and fruits from May to July of V. amurensis from Zhonggong Nanshan of Jinan. After washing with water, cutting, drying in the shade, powdering, sieving through 40 mesh, and extracting by the best extraction method, the formation and content of resveratrol were assayed by liquid chromatography-mass Spectrometry. The results showed that there was trans-resveratrol in V. amurensis, and its content was the highest in roots and stems in September, and it is highest in red pericarp compared with others. It also suggested that the content of resveratrol was different in different parts of V. amurensis and varies in different seasons.