Study On Ultrastructure Of Cells And The Expressions Of Mast Cells And IL-2,IFN-γ On Colonic Mucosa In Patients With Post-infective Irritable Bowel Syndrome

Objective:(1) To investigate whether there are changes of the mast cells(MCs) and the Thl cytokine(IL-2,IFN-γ),and to elucidate their possible roles in visceral hypersensitivity in patients with irritable bowel syndrome(IBS).(2) To investigate whether there are changes on ultrastructure of cells in colic mucosa and to elucidate their possible roles in visceral hypersensitivity in patients with irritable bowel syndrome.Methods:In 10 normal controls,30 patients with post-infective irritable bowel syndrome(IBS),biopsies were taken from the sigmoid colon.The MCs and IL-2,IFN-γwere stained by histochemical and immunohisto-chemical method respectively, and color image analyzer investigated the results qualitatively and quantitatively.The ultrastructure of cells in colic mucosa and the changes of the mast cells(MCs) were studied through an ultra-microscopy using in situs embedding technique and a light microscopic study in serial sections.Results:(1) The number of MCs in sigmoid colon was significantly elevated in IBS(p＜0.01),and) MCs in IBS have prominent variations.Significantly increased the IL-2 and IFN-γwere found in intestinal mucosa of IBS compared to the control. (2) The number of MCs in sigmoid colon were significantly elevated in IBS,and MCs in IBS have prominent variations.The colic mucosa showed the integrity of epithelial cell membrane,normal cell shape,the irregular distribution of microvilli of different length,and the cytoplasmic organelles including mitochondria,rough endoplasmic reticulum etc.have obvious change.The aperture between cells were remarkably spacious.A large number of secretory granules,vacuoles,rough endoplasmic reticulum etc.were seen in the cytoplasm of goblet cells,plasmocyte, mast cell and neuroendocrine cells.Conclusions:(1) Expression of MCs and IL-2,IFN-γwere significantly increased in post-infective-IBS.That means the activation of colonic mast cells(MCs)is involved in the visceral sensitization.The mast cells(MCs) may be the middle link of immunity mechanism and nerve mechanism.But twos have no relativity.(2) There are obvious changes on ultrastructure of cells in colic mucosa.That means IBS may be a gastrointestinal organic disease with ultrastrural alterations in colonic mucosa.。...