Protective Effects Of Total Polysaccharides From Yu-Ping-Feng Powder On Acute Liver Injury And Study On Its Mechanism

The acute chemical and immunological liver injury models were established,We investigate its protective effects and explore partly mechanisms of total polysaccharides from Yu-Ping-Feng Powder(YPF-P) in acute liver injury mice.The main results are as follows:1.Protective effects of YPF-P on CCl4-induced chemical liver injury mice and its partly mechanisms.YPF-P(200 mg·kg^<sup>-1,ig)could significantly reduce the liver index and the elevated ALT,AST activities in serum;YPF-P(100,200 mg·kg^-1,ig) could decrease MDA contents in liver homogenates,enhance activities of SOD and GSH.All of these suggest that YPF-P has protective effects on CCl₄-induced chemical liver injury.It is possibly related to the reduction of cytokines and inhibition of lipid peroxidation.2.Protective effects of YPF-P on D-Gal-induced chemical liver injury mice and its partly mechanisms.YPF-P(200 mg·kg^-1,ig) could significantly reduce the elevated ALT,AST activities in serum and MDA contents of liver homogenates,enhance activities of SOD and GSH in liver of D-Gal(800 mg·kg^-1,ip)-induced chemical liver injury mice.The pathology results of liver also show that YPF-P has liver protection to a certain extent. These results suggest that YPF-P has protective effects on D-Gal -induced chemical liver injury.It was possibly related to the inhibition of lipid peroxidation. 3.Protective effects of YPF-P on acute immunological liver injury mice and its partly mechanisms.YPF-P(100,200 mg·kg^-1,ig) could significantly reduce the elevated ALT,AST activities in serum and MDA,NO contents of liver homogenates,enhance activities of SOD,GSH,and inhibit production ofTNF-α,iNOS,TNF-αmRNA in liver of acute immunological liver injury mice.The dimensions of hepatic damage in the histopathology had reduced.These results suggest that YPF-P has protective effects on acute immunological liver injury.It is possibly related to removing free radicals, enhancing the antioxidant capacity of lipid peroxidation and reducing the expression of inflammatory cytokine.