The Preliminary Study Of The Biocompatibility Of Hydroxyapatite Material And Human Cord Blood Endothelial Cell

Background: The incidence of heart valve diseases is very high, valve replacement is an effective way for the treatment of heart valve diseases internationally. The mechanical valve is the most extensive artificial heart valves in the clinical application of valve replacement, but one of the greatest problems is the need for life-long anticoagulant therapy. Otherwise, they will be will happen thrombosis on the valve and the vicinity of the valve, it would impact the valve switching functions, and cause embolization of important organs. Because of improper taking anticoagulant drugs, some patients will suffer hemorrhage, embolism, function failure of mechanical valve, or even heart failure and other complications. So to solve the mechanical valve coagulation problem is the core content to the application of mechanical valve.Objective: To investigate biocompatibility of the hydroxyapatite materials (Hydroxyapatite, HA) with vascular endothelial cells, and to provides an ideal film material for the application of tissue engineering mechanical valves.Method: Part I: The cell line of human umbilical cord vein endothelial cell was co-trained with hydroxyapatite materials in vitro. We are going on morphological observation and the growth curve determination, MTT assay, and protein content analysis. Part II: human umbilical vein endothelial cells were implanted on the surface of hydroxyapatite materials, through the test of the adhesion of platelets, blood coagulation, and dynamic blood clotting test to detect blood compatibility.Result: (1) human umbilical vein endothelial cells have a good adhesion, growth, and proliferation on hydroxyapatite materials. The generation of 2, 4, 6 cell growth curves are basically the same, the cellular proliferation is fast. MTT assay and Lowrry test showed that HA materials has no adverse impact on the proliferation activity of human umbilical vein endothelial cells. (2) The umbilical vein endothelial cells cultured on the surface of hydroxyapatite materials one week, then did platelet adhesion experiment, whole blood coagulation and dynamic blood coagulation experiments indicated that the the anti-clotting ability of human umbilical vein endothelial cells covered HA is superior to simple artificial mechanical heart valves and hydroxyapatite only.Conclusion: The hydroxyaptite material has good cell compatibility and blood compatibility, can be safely applyed as tissue engineering and mechanical valve source material.