Experimental Study Of Diamond-like Carbon Blood Compatibility And Human Umbilical Vein Endothelial Cells Seeding

Background:With the large number of medical polymer materials applied in the biomedical field, such as tissue engineering, organ transplants, etc, modern medical diagnosis and treatment means have been enriched. Valvular disease is a commonly- seen heart disorder,valve replacement is an effective approach to treat valve disorders. Both the mechanic and bioprosthetic valves used now have their own defects,such as inability to grow and autorepair,need for anticoagulation,susceptibility to infections , making it important for the cardiac surgery field to develop some kind of ideal valves.With the development of tissue engineering techniques,the tissue engineering valves are thought to have great potential and bright clinical future,and now it has become a hot point in cardiac surgery.However, many problems of tissue engineering valves have not still been resoved, such as making stent, cellular implantation and cultivation of the valve in vitro. Hence it is urgent that we researched heart valve with a good biocompatibility and durability.Objective: Investigate Diamond-like carbon films blood compatibility and observe the growth of human umbilical vein endothelial cells on the DLC films surface.To discuss the biocompatibility of DLC films and HUVEC. To obtain ideal cardiovascular medical materials.Methods: Selected human umbilical vein endothelial cells in log growth phase, diluted to 1×104/ml ,then inoculated them to 96- pore plates with diamond-like carbon films, set up negative control group (blank group),and cultivated them in 37℃constant temperature incubator with 5%(V/V)CO2. Took out a plate everyday in 24h,48h, 72h,96h and 120h.Detected A value of cells with MTT. according to the standard curve ,calculated the number of cells everyday and drew cell growth curve.Took out a plate in 3,5 and 7 days and scanned under SEM.Contrasted with the negative group and determinated material anticoagulative activity in vitro with APTT, PT and TT.Results: From endothelial cell growth curve drew by MTT we can saw that when cell cultured together with materials , cell number was increasing with the passage of time. Compared with negative control group, there were not significant influence in cell proliferation. Cellular connection was found by scanning electron microscope with the prolongation of cultural time.On the material anticoagulative activity,diamond-like carbon films did not cause PT, APTT and TT change significantly,which may have good blood compatibilityConclusion: Cell proliferation has not been significantly affected in the DLC thin film material, PT,APTT and TT have no significant changes .The compatibility of DLC and HUVEC is good and blood compatibility may be good. The material may be safely used in heart valve construction.