The Study On The Effecting Of Astragalus And Other Chinese Medicine On Multiplication Of Bifidobacteria

Bifidobacteria are strict anaerobic germs, hard to nutritional condition, not tolerant to low pH, and short survivibility. This resulted in the great difficulty for Bifidobacterium fermenting dairy products in industrialization. aiming at the peculiarity of Bifidobacterium , the tasks studied the effects of Bifidobacterium Longum and Bifidobacterium bifidus's extract on the growth of Bifidobacterium in order to provide theeoritical foundation for the utilization of Astragalus mongholicus Bunge resource ,using Astragalus mongholicus Bunge as experimental material and Bifidobacterium as strain tested.This experimental selection's Chinese medicine has the health care function drugs for the medicine food homology or to the human body, used the turbidimetric method and Hungate anaerobic rolls the tube technology counting to select the Astragalus which from many kinds of Chinese medicine a promotion of Bifidobacteria growth. The influence experiment which grows to Bifidobacteria indicated through the Astragalus in vitro that the Astragalus leaching solution has the promoter action in vitro to Bifidobacterium Longum (Blm) and the two things which do not tally Bifidobacterium bifidus (Bbm) growth, its effect increases along with the Astragalus leaching solution recruitment increases, and presses the growth effect existence difference to the different mold mushroom spawn. Has studied take the Astragalus leaching solution as the primary data Bifidobacterium culture medium, the massive test result indicated that increases ingredients and so on soy protein peptone, pancreas protein peptone, cow meat extract, corn thick liquid and dipotassium hydrogen phosphate in the Astragalus leaching solution, has the obvious promotion growth function to Bifidobacterium, using the Astragalus leaching solution, the soy protein peptone, the corn thick liquid and the cow meat extract has designed four factor three level orthogonal tests, had determined the Astragalus compound increases the fungus culture medium the optimized formula: 12.5% radix astragali leaching solutions, beef extract0.6%, corn thick liquid 0.4%, maize hydrolyzates 0.5%, K₂HPO₄0.2%. and the live counts cultivated in the optimum enrichment medium for 16h at 37℃were 4.59×10⁹ cfu/mL of Blm and 2.91×10⁹ cfu/mL of Bbm.Through one selective Bifidobacterium culture medium, take the healthy white mouse as the experimental subject, studied the radix astragali in the animal intestinal tract to Bifidobacterium 's influence, the animal experimentation result had indicated that the radix astragali leaching solution had the selectivity promoter action in vivo to Bifidobacterium 's growth, but in the intestinal tract the main pathogenic bacteria backwoods coli's growth received suppresses, in the radix astragali multi-carbohydrate material possibly grew to the radix astragali at in vivo selectively promotion Bifidobacterium the leading role, in addition, the radix astragali leaching solution also had the obvious entire intestines function, compared with the control group, feeds the radix astragali leaching solution the mouse bowel movement to be smooth, the excrement pellet was big and is moist.