Astragalus Extraction And Separation Of Effective Components And Structural Identification

The radix astragali is the leguminous plant,is used as medicine by the root.For many years continuously take AstragalosideⅣas its quality control target,The Pharmacology findings indicated,In the radix astragali produces the drug efficacy effective component also to have the radix astragali flavanone,saponin and polysaccharide and so on。This topic to enhance these three kind of chemical composition the extraction rate,Uses the cellulose hydrolysis first to process the radix astragali,Then carries on the radix astragali flavanone,the saponin and the polysaccharide extraction separation again,Has served the anticipated experiment purpose。Research definite experimental result:Used the orthogonal experiment method to determine in pH4.6,the cellulase amount used 0.8%,temperature 60℃,under the enzymolysis 120min condition,carried on the extraction before the radix astragali to process, causes the radix astragali polysaccharide,the total flavanone and the total glucoside extraction rate has the large scale enhancement.And the radix astragali polysaccharide's extraction rate compared the enzymolysis radix astragali not to enhance above 300%。After enzymolysis radix astragali uses 80%ethyl alcohol backflow extraction process to withdraw the total flavanone and the total saponin,uses the combination isolation technique to carry on the separation purification again:After polymide adsorbs the total flavanone the water eluent to flow through the D101 macroporous resin adsorb the total saponin,and the separation to purify the total flavanone and the total saponin,the two's extraction rate is 4.79%and 1.26%, the purity is 58.0%and 79.3%。using the lye solution from the total saponin to obtain two compound,was AstragalosideⅢand AstragalosideⅣ。Wield the completely ethyl alcohol after the ethyl alcohol extraction's radix astragali dregs of medical decoction,joins the distilled water according to the liquid-solid compared to the 1:11 quantity,the backflow withdraws 3 times,each time 75 min,the total sugar extraction rate is 33.6%.Uses the ultrafiltration partition collection radix astragali total polysaccharide,its molecular weight is bigger than 50000Da the polysaccharide to approximately compose 12.23%,accounts for 68.25%between the 10000～50000Da polysaccharide,while is smaller than 10000Da accounts for 19.52%.