| ObjectiveThis study aimed to investigate the expression of Chemerin and its receptor ChemR23 in lung cancer and to explore their functions in the tumorigenesis and progession of lung cancer.MethodsThe expressions of Chemerin and its receptor ChemR23 in lung cancer tissues and normal lung tissues were tested by western blot. Then the location of Chemerin and ChemR23 in lung cancer tissue were investigated by immunochemistry. Also the expressions of Chemerin and ChemR23 in lung cancer cell A549 and normal lung cell WI-38 were tested by RT-PCR. With their primer, Chemerin and ChemR23 cDNA was cloned from normal lung cell WI-38, and mammalian recombinant plasmids containing Chemerin and ChemR23 cDNA was constructed, named pCDNA3.1-Chemerin and pCDNA3.1-ChemR23, respectively. Then lung cancer cell A549 was transfected with these recombinant plasmids. The proliferation assay, migration assay and invasion assay were made with Chemerin transfected A549 cell. On the other hand, NF-kB, JNK and P38 were tested by western blot in A549 cell with ChemR23 over expressed.ResultsWestern blot result showed that the expression of Chemerin is lower in tumor than in normal lung tissue. The expression of ChemR23 have no obvious difference between tumor and normal tissue. Both Chemerin and ChemR23 are located in cancer cell. Also we found that Chemerin mRNA is silenced in lung cancer cell A549, but constitutively expressed in normal lung cells by RT-PCR. The abilities of proliferation and invasion in A549 cell with Chemerin over expressed in vitro have no change, compared with lung cancer cell A549 stably transfected with empty plasmid. Chemerin can obviously enhance movement ability of A549. Classical NF-kB pathway was activated and P38,JNK pathway were inhibited in A549 cell with ChemR23 over expressed, compared with A549 transfected with empty plasmid.ConclusionsChemerin-ChemR23 system probably play a key role in the tumorigenesis and progession of pulmomary carcinoma. |
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