The Relationship Between Chemerin/ChemR23 And Inflammatory Factors In Diabetic Nephropathy

ObjectiveChemerin is a kind of peptides with chemotaxis, combining with its main ligand ChemR23,inducing ChemR23 specific cell aggregating, so as to play a number of important role.Diabetic nephropathy (DN) has become the leading cause of end-stage renal disease, there is no effective treatment measures so far.Chemerin and its ligand ChemR23 are closely associated with inflammation which plays an important role in the process of diabetic nephropathy. This study was to observe change of the Chemerin/ChemR23 expression level and associated with inflammation in kidney and serum in the progression of diabetic nephropathy rats, especially in the early diabetic kidney injury.Methods80 male SPF level Sprague-Dawley (SD) rats(160-180) g were randomly divided into normal control group(n=40) and diabetes group(n=40). Rats from diabetes group were given a single intraperitoneal injection of 75 mg/kg streptozocin (STZ) solution, while the others were given a single intraperitoneal injection of 75 mg/kg citrate buffer. At the end of one week, two weeks, three weeks, four weeks and twelve weeks after STZ injection, body weight, kidney weight, blood glucose, UACR,SCr and change of renal pathology was detected to evaluate if diabetc nephropathy model was successfully established. Diabetes without proteinuria group, diabetes with microalbuminuria group and diabetes with massive proteinuria group was choosen.What’ more, Chemerin and ChemR23 in kidney was detected, Chemerin in serume was detected, which belong to these groups. At last, correlational analysis was conducted between Chemerin and ChemR23, TNFα, IL6 and CRP.Results1、DM group of SD rats appeared gradually obviously polydipsia, polydipsia and polyphagia, dark colour, activity decreased significantly, after injection of STZ after 72 hours,and random blood glucose was over 16.7mmol/L for three days, which indicated diabetes model success. With the progress of diabetes, rats’ weight decreased and blood glucose, kidney weight index, the urinary albumin/creatinine ratio increased significantly.4 weeks after diabetes model was successfully established, the rats appearanced microalbuminuria, urinary albumin/creatinine ratio increased from (0.36±0.22) mg/mmol (the normal control group) to (1.46±0.49) mg/mmol (diabetic nephropathy group). Renal pathology also suggest diabetic early renal damage model successfully reproduced.12 weeks after diabetes model was successfully established, rats appeared a large number of albuminuria, urinary albumin/creatinine ratio rase to (2.64±1.11) mg/mmol, serum creatinine increased significantly, and renal pathology also suggested experimental animals had moved into the massive proteinuria stage of diabetic nephropathy.2、Early on the stage of diabetic kidney damage, the experimental group rats started to appear albuminuria, Chemerin and ChemR23 expression level in kidney increased significantly, and increased with the progress of the disease.Spearman correlational analysis showed, protein expression levels of Chemerin and inflammation factors including TNFα,IL6, CRP in serum were positively correlated; meanwhile, mRNA expression levels of Chemerin and ChemR23, inflammation factors including TNFα, IL6 in kidney were positively correlated.ConclusionChemerin and ChemR23 could be detected in the kidney tissues and serum in the time of diabetes involving kidney, with further increases in disease progression, and Chemerin’s expression was positively correlated with inflammation factor such as TNFa, IL6 and CRP in DN.