The Study On Toxicology And Pharmacodynamics Of Supramolecular Extract From Andrographis Paniculata (Burm.f.) Nees. And Its Anti-inflammatory Mechanisms

Andrographis paniculata(Burm.f.)Nees. is a traditional Chinese drug,Supramolecular extract of Andrographis paniculata (Burm.f.) Nees. (SEA) is a new preparation which developed by Natural Drug Institute of Henan University recently. The Andrographis Paniculta is one kind of herbal drug for relieving the internal heat of the body, it has the effect of ant febrile, detoxifies,cooling blood and detumescence, so it is used to treat the heat-syndrome of the interior in the differential diagnosis.,in the medicinal works"the herbalism of Quanzhou", there was the records for this medinical plant that it may lower febrile, detoxify, diminish inflammation and tumefaction, may be used to the throat inflammation, desentery and high fever. The previous pharmacological research indicated that the Andrographis Paniculta preparation have strong action in antipyretic, antiphlogistic, anti-bacterial and anti-viral. therefore it is widely used in clinical applies: it is applied to the fever of cold, acute and chronic pharyngitis, dysentery, ndotoxemia and so on.Objective:In this research we designed the acute toxicity and the main pharmacodynamics research of SEA preparation , and its anti-inflammation mechanism in order to offer pharmacological and experiments basis for its safe and effective use in clinic.Methods:There are three parts in this thesis:PartⅠAcute toxicity research on SEAWe observed from the pre-experiment of acute toxicity research on SEA that median lethal doses (LD50)of SEA is 717.8mg·kg-1.partⅡMain pharmacodynamics research on SEA1.Antipyretic experiments The dose of 600mg·kg -1and 300mg·kg-1 of SEA,keep on three days,can be significantly decreased high temperature induced by dried yeast in rat or 2, 4-dinitrophenol and compared to the model control group have significantly difference (p <0.01 and p <0.05).2. Anti-inflammatory experimentsThe dose of 600mg·kg-1 and 300mg·kg-1 of SEA,keep on three days ,can be significantly decreased ear edema induced by dimethylbenzene in mice, effect on granuloma hyperplasia in rat, effect on paw swell induced by egg white in rat and mouse peritoneal inflammation induced by Acetic acid. compared to the model control group have significantly difference(p <0.05).PartⅢAnti-inflammation mechanism research on SEAThe dose 600mg·kg-1 and 300mg·kg -1of SEA for three consecutive days administration can be significantly inhibited contents of PGE2 and MDA,enhance the activity of SOD and CAT.Results:1. Acute toxicity tests showed that SEA have smaller acute toxicity, it is safe for clinical use.2. In this study,we choose SEA as the new study, A major study on its anti -inflammatory and antipyretic effects, and compared with the market Chuanxinlian agents. The results showed that SEA have a significant anti-inflammatory and antipyretic effects, and have the same pharmacological effects to the same dose of Andrographolide agents.3. SEA inhibiting inflammation at the same time,could significantly inhibit the formation of PGE2,reduce the generation of MDA, enhance the activity of SOD and CAT. Results suggest that, anti-inflammatory mechanisms of SEA may related to inhibiting the formation of PGE2,reducing the generation of MDA, enhancing the activity of SOD and CAT.