Experimental Study On Manufacturing And Evaluating The Neonatal Rodents Model Of Cerebral Hypoxia-ischemia

Objective To explore the method of making hypoxia ischemia model with neonatal rat and evaluating it.Method Seven days old healthy Wistar rats was used in this study, after the right common carotid artery was ligated and mutilated,the rats was put into a airtighted box with the volume fraction of 8%oxygen and 92%nitrogen.At different time point,Balance Beam Test,Footprint Analysis Test,Motor Evoked Potential and Pathology Examination are performed.Result(1)the Footprint repeat space show that in the HIE group rat's left limb footprint repeatability is poor than the control group at 3 weeks and 7 weeks after operation.In HIE group the rats' space between the footprints is larger and instability,significantly larger than the control group(P＜0.05);(2) In the Balance Beam Test,the time used by the HIE group rat to cross the balance beam is longer than the control group.The frequency of rats' lelf leg slip from the beam was significantly more than the control group.(P＜0.05);(3) At 3 and 7 week after operation,the HIE group rats's hindlimb quadriceps CMAP draws out the wave amplitude to be low compared with control group(P＜0.05);(4) General observation show the right brain volume become analosis and smaller than the contralateral.HE stain shows that the cells aroud impaired cerebral ventricleperiventricular is swelling,and cell number less than the contralateral.The cortex neurons decreased,degeneration and necrosis significantly at the impared side,Glial cells reactive hyperplasia.Conclusion Through ligate the unilateral carotid artery and hypoxia,the cerebral palsy model we obtain is stable and reliable,And can be used for experimental research related to cerebral palsy.Neuroethology and electrophysiology have the very big application space in the cerebral palsy animal model appraisal aspect.