Establishment Of Spastic Cerebral Palsy Model Of Spastic Cerebral Palsy By Partial Resection Of Motor Cortex

Objective To apply partial resection of the macaque motor cortex in constructing young hemiplegia macaque models and evaluation methods.Methods 4 individuals of 3-month-old macaques were randomly divided into normal control group and operation model group.Partial resection of the motor cortex was carried out in operation model group;precentral gyrus cortex from above the right lateral cerebral fissure to the inter-hemicerebral fissure,together with the posterior-superior frontal gyrus(about 0.3 to 0.5cm in front of the anterior median sulcus)cortex was removed to the depth of about 0.5 to 0.6cm.In order to observe the dyskinesia of contralateral limb and the abnormal dynamic-static posture of different individuals,we used video to record the consecutive behaviors after the operation.We also used manual test and modified Ashworth scale to examine the manifestations of human spastic hemiplegia such as muscle tone abnormality,quantitative index of gross motor and fine application of self motion assessment test two group of movement disorders further that movement disorder operation model group,using 9.4T MRI to detect brain imaging changes.Results(1)The operation model group after operation immediately left hemiparesis,left upper limb abnormal lifting,left lower limb paralysis,left limb claudication,eat mainly rely on the right side of the body.The activity of the operation group was significantly lower than that of the normal group at one week after operation,and the score was lower than that of the normal group;the activity increased after 2 to 3 weeks,and the score also began to rise,until after the 8 week,the score was stable.The gross motor operation model group and normal control group in the postoperative score of each period is not exactly the same(Friedman test,?2= 33.939,P < 0.05),fine motor score of different periods after operation(Friedman test?2= 37.526,P < 0.05).(2)The operation model group,abnormal posture mainly tilted to the left for the rest,sitting in a corner of the monkey cage,left arm up on the cage online to maintain postural balance,movement is left inclined,which had simulated the typical clinical manifestations of human spastic hemiplegic cerebral palsy.(3)We used manual test to examine the changes of muscle strength and muscle tone: the operation group had mildly reduceed left muscle strength after operation,left muscle tension gradually began to increased after 5 weeks,and gradually increase to 3 grade,which has continually increased to now.The muscle tension on both sides of the operation model group in 5 weeks after surgery and postoperative 14 weeks after each period is not exactly the same(Friedman test,?2= 20.713,P < 0.05).(4)Brain MRI 3 weeks postoperatively suggested scar tissue formation after right motor cortex resection,which supported the pathological changes of the hemiplegic cerebral palsy models.Conclusion(1)Partial resection of unilateral motor cortex allows for successful construction of young spastic hemiplegic cerebral palsy macaque models.(2)Applying video into observing the dyskinesia and abnormal posture of individual macaques,and manual test in examining changes of muscle strength and muscle tone,together with self apply macaqueneurobehavioral score and changes of cerebral imaging allowes for accurate evaluation of constructing the cerebral hemiplegic palsy macaque models.(3)Accurate simulation of infant cerebral traumatic spastic hemiplegic palsy model has provided a scientific platform for research on pathological mechanism and clinical rehabilitation of cerebral palsy.