The Role Of Dendritic Cells In The Experimental Mouse Of Model With Vaginal Candidiasis

ObjectiveTo investigate the variations of CD11c positive dendritic cells (DC) in vaginal mucous and the expression of costimulatroy molecules on the surfaces of DC from draining lymph node and spleen in mouse with experimental vaginal candidiasis.MethodsFemale 8 to 10 weeks of age mouse(ICR), Studies with an estrogen-dependent mouse model of vaginal candidiasis . The mouse were killed at 2nd day,4th day,7th day,14 th day,21 th day after inoculation ,respectively. The vaginal lavage fluid,the vaginal tissues,the spleen cells and the draining lymph node cells were obtained at day after the mouse were killed . The variations of positive DCs in vaginal mucous was detected by immunohistochemistry. FACS was used to measure the expression of CD80,CD86 on spleen-derived dendritic cell and draining lymph node derived DC.ResultsThere was high load C.albicans in the culture of lavage fluid of inoculation candidiasis group from the second day to the end of experiment. Vaginal tissue from C.albicans inoculated mouse contained a lot of hyphae and inflammatory cells in the superficial mucosa. The average optic density of CD11c positive DCs in vaginal mucous in inoculation candidiasis group was higher than that in control group. (P < 0.05) Compared with that control, the expression of CD86 on DCs from draining lymph node is rapidly upregulated after activation, whereas CD80 has a slower kinetics of upregulation. There was no difference in expression of CD80,CD86 on spleen-derived DCs.ConclusionOur results suggest that candidiasis infection caused the over expression of CD11c+ dendritic cells of vaginal mucous in mouse. Morever, via upregulation of CD86, DC might play a pivotal role in host defense mechanism in the mouse model of experimental vaginal candidiasis.