Effects Of Ca～(2+) On The Proliferation And Differentiation Of Bone Marrow Mesenchymal Stimulated By Electromagnetic Fields

Objective To study the effects of Verapamil on the proliferation and differentiation of bone marrow mesenchymal stimulated by electromagnetic fields(EMFs) of 50Hz frequency and 0.8mT intensity.Methods he mouse bone marrow mesenchymal stem cells were isolated and cultured in vitro, The fourth-passage cells were harvested and divided into A,B,C and D groups effected by EMFs and Verapamil. Application of the MTT colorimetric assay to measure the cellular proliferation and the activity of ALP was measured according to the approach which the biological company suggests. The modified Gomori staining was made too.Results EMFs of 50Hz frequency and 0.8mT intensity induced a significant increase of cellular proliferation (P<0.01) and inhibited the activity of ALP(P<0.01) in comparison to the controls, but Verapamil inhibited the increase and enhanced the activity of ALP.Conclusions EMFs of 50Hz frequency and 0.8mT intensity changed intracellular free calcium ion concentration of bone marrow mesenchymal stem cells, and the change play a key role in the celluar proliferation and play a partial role in the differentiation of bone marrow mesenchymal stem cells.