Studies On Chemical Constituents In The Bile Of Anser Anser And Its Pharmacological Activities

In this paper, the chemical compositions and pharmacological activities of the bile of Anser anser were studied.According to the references, many kinds of compounds were isolated and identified as so far from animal's bile, such as bile acid,bile pigment,cholesterol,fatty acid,minor element,amino acids and so on. The modern pharmacological studies showed that bile had the effects of antibiosis ,antiinflammatory,antioxygen, anticancer etc.. In order to find out more chemical constituents which have bioactivities in the bile of Anser anser and develop more new healthy maintenances and drug products, our group gave a comprehensive researches. The research methods and results are as follow:Firstly, pulverized and dried bile of Anser anser (500g) were extensively extracted with 85% alcohol and the combined extract were concentrated in vacuo until `no-alcohol, then got the total extracts (45g), Which were extracted further by petroleum ether, chloroform, acetic ether, and n-butyl alcohol respectively at room temperature. By using silica gel column chromatography,TLC and recrystallizing methods 10 compounds were obtained from extracts. Among of them, seven compounds′structures were established and identified on the basis of chemical and spectroscopic evidence (1HNMR,13CNMR,2DNMR, MS).They are phenylacetic acid (Ⅰ),Chenodeoxycholic acid (Ⅱ) , 3α,7α-dihydroxy- 5β-cholan-24- carboxylic ethyl ester (Ⅲ), 3α-acetoxy-7α-hydroxy-5β-cholan-24-oic acid(Ⅳ), (Z)-6-Octadecenoic acid (Ⅴ), hexadecanoicacid , 2,3-dihydroxypropyl ester(Ⅵ), (4E)-2-[2′-hydroxyhexadecanoylamino]-4-Octadecane-1,3-diol (Ⅶ).The inhibiting Matrix Metalloproteinase biological activities of the compoundⅡhave been study firstly. The result showed that it had a clear effect of inhibiting Matrix Metalloproteinase.