| Objective: In recent years, along with the extensive application of extracapsular cataract extraction (including phacoemulsification), after-cataract(AC) has already become the most common complication after the surgery of cataract. At the adult, the incidence rate of AC is 30%~50%, which has already become the important factor affects the postsurgical visual recovery of cataract patients, at the child then is 100%. It is believed that the leading mechanism of AC is the proliferation, the transplatation, the transmigration and the transformation of the residual lens epithelial cells(LEC), which causes posterior capsular opacification(PCO). It will have the huge clinical significance if an effective drug which is beneficial to prevention of PCO was produced. Curcumin(Cur), is one kind of phenol pigment which is extracted from the rhizome of Curcuma longa, widely applies in the food additive, the dye and the spice. In current years, the massive elementary and clinical experiments indicate that Cur has various medical function, such as anti-inflammation, antioxidant, anti-coagulation, anti-viral and inhibition on proliferation of various tumor cells. In our experiment, we study on the inhibitiory role of Cur on proliferation of human LEC cultured in vitro and the relationship between dose-effect and aging, in order to achieve the clinical purpose of preventing PCO.Method: (1) To build a human LEC line which was good-morphous and well-functioned, and select the cells which were during log phase growth as the experimental object. (2) 0.01,0.02,0.03mg/mL Cur were added to the LECs cultured in the orifice separately for 24 hours, and 5%1640 was added to the blank. The proliferation of human LEC which was cultured for 12,24,48 huors after addition of 0.02mg/mL Cur was detected by methyl thiazolyl tetrazolium(MTT). The proliferation of human LEC was detected by MTT after addition of 0.02 mg/mL Cur for 12,24,48hours. (3) 0.01,0.02,0.03mg/mL Cur were separately added to the LEC cultured in the cell culture bottle separately for 24 hours, and 5%1640 was added to the blank. The expression of proliferating cell nuclear antigen(PCNA) in human LEC was analyzed by flow cytometer(FCM). The expression of PCNA in human LEC was analyzed by FCM after addition of 0.02 mg/mL Cur for 12,24,48hours.Result: (1) After addition of 0.01,0.02,0.03mg/mL of Cur for 24 hours, the inhibitory ratios of proliferation of human LECs were 18.45%,39.01% and 69.98%, respectively. It shows that Cur has obvious inhibitory effect on proliferation of human LECs, and the more concentration the larger inhibition ratio is. LECs were treated with 0.02mg/mL Cur for 12,24,48hours, the inhibitory ratios of proliferation of human LECs were 27.62%,39.01% and 60.72%, respectively. It shows that the longer time that LECs were treated with Cur the larger inhibition ratio is. (2) After addition of 0.01,0.02,0.03mg/mL of Cur for 24 hours, the positive ratios of PCNA staining were 57.76%,41.08% and 32.82%, respectively. It shows that the more concentration of Cur the saller ratio of PCNA staining, that is, larger inhibition ratio of human LECs. LECs were treated with 0.02mg/mL Cur for 12,24,48hours, the positive ratios of PCNA staining were 55.06%,41.08% and 25.96%, respectively. It shows that t the longer time that LECs were treated with Cur the saller ratio of PCNA staining, that is, larger inhibition ratio of human LECs.Conclusion: The proliferation of human LECs is conspicuously inhibited by Cur. The Cur may become a potential drug to prevent and cute after cataract. |
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