The Effects Of Bone Marrow Mesenchymal Stem Cells(MSCs) And Basic Fibroblast Growth Factor (bFGF) Combined With Degrading Membrane On Repair Of Skin Defect

Title: the effects of bone marrow mesenchymal stem cells(MSCs) and basic fibroblast growth factor (bFGF) combined with degrading membrane on repair of skin defectObjective: To investigate the effects of MSCs and bFGF combined with degrading membrane (Gelatin-Chitosan-Polyviol membrane) on the repair of full-thickness skin defect.Methods: MSCs were drew from fifteen New-Zealand rabbits, and isolated, cultured, amplicated, cloned and purified. Make three round raw surfaces with 1 cm radius on the back of every rabbit, removal full-thickness skin and subcutaneous tissue and deep to fascia profunda. The raw surfaces were randomly divided into A, B and C three groups. A group: calcium alginate gel containing MSCs + bFGF combined with degrading membrane(Gelatin-Chitosan-Polyviol membrane) + mniotic membrane; B group: calcium alginate gel containing MSCs + mniotic membrane; C group: calcium alginate gel without MSCs + mniotic membrane. The isolated and purified MSCs were planted into raw surfaces at same concentration. On the 7th day, the 14th day and the 21th day after implanting, the condition of wound healing were observed by gross observation, routine histological observation (HE staining), type I collagen immunohistochemistry observation and electron microscope. To compare the epidermal thickness, dermic thickness and the number of collagenoblast of wound by image analysator, the condition of wound healing of every groups in different time. Results: (1)Nascent dermis tissues in group A were obviously thicker than those in group B, collagenoblast with vigorous function, especially type I collagen positive collagenoblast, blood vessels and collagen fibers in group A were more than those in group B in different time (7 d, 14 d and 21 d). The all above targets in group B was obviously better than that in group C. (2)Growth velocity of nascent epidermis in group A was obviously faster than that in group B on the 14th day and the 21th day after implanting. Areas around nascent dermis tissues in group A were more than that in group B, especially on the 21th day after implanting. Nascent epidermis of group A already mostly reconstructed into multiseriate epidermis construction and the conditions of rebuilding were obviously better than those in group B and the conditions of rebuilding in group B was also obviously better than those in group C. (3)The wound epidermal thickness, dermic thickness and the number of collagenoblast by image analysator in different time were better in group A than those in group B and group C. All the experimental data were statistically treated and following results were gained by analysis of variance (ANOVA).Conclusion: Bone marrow mesenchymal stem cells (MSCs) and basic fibroblast growth factor (bFGF) combined with degrading membrane (Gelatin-Chitosan-Polyviol membrane) has the effects of promoting dermic regeneration and repair, which will supply a new choice for searching ideal tissue engineering skin. During the research of tissue engineering skin, we should pay close attention to the improvement of microenvironment in the transplant places.