| Objective: Reperfusion after acute myocardial infarction (AMI) is the main treatment to acute myocardial infarction. The mechanism of left ventricular remodeling and how to control it have become a hot issue. Allopurinol alcohol (Allo) is xanthine oxidase (XO) inhibitor. Studies found that: Allo can protect heart from ischemia-reperfusion injury, which caused the concern of scholars at home and abroad for a long time. The role of Allo to prevent left ventricular remodeling is seldom reported. This experiment is to observe the treatment ation of Allo on myocardial ischemia reperfusion models, the aim is to explore the effection of Allo on left ventricular remodeling in myocardial ischemia reperfusion (MIR) rats.Method: Rats were randomly divided into sham group; MIR group and Allo treatment group. While models are successfully producted, then medicines are administrated through gavage for at least four weeks. We observe the left ventricular remoding from 3 directions specimen; light microscopy; electron microscopy, and then by testing plasma endothelin (ET), serum superoxide dismutase (SOD), malondialdehyde (MDA), nitric oxide (NO) content, and other indicators.Results: First, ventricular remodeling indicators contrast results:Allo group compared with the MIR, left ventricular relative weight and septal thickness are significantly reduced. Second, Light microscopy results: sham group myocardial fiber, myocardial cells, myocardial interstitial are no obvious abnormalities. Myocardial fiber of MIR Group arrange disorder. Myocardial cells shows necrosis. myocardial interstitial are obviously edema, Allo group than MIR Group is to reduce ventricular remodeling.Third, electron microscopy results: myofilament, sarcomere, mitochondria of sham group are no obvious abnormalities. Muscle tow of MIR Group have fracture and dissolution partly. The mitochondria MIR Group become smaller, and have cavitation. Myofilament of Allo group arrange in order, sarcomere shad with clear, and have cavitation.Forth, biochemical indicators comparative results: There are less ET,MDA in Allo group than MIR Group, but more SOD,NO in Allo group than MIR Group.Discussion: The experimental results and the pathology specimen of this study reflect that MIR injury model is successfully established.The oxygen free radicals are generated after AMI, blood supply and oxygen supply are restored after reperfusion. A large number of oxygen free radicals (OFR) are generated, SOD consumption increased. NO synthesis and ET secretion will result in chronic and acute injury and lead to cell injury or death, then the occurrence of ventricular remodeling is exacerbated. Compared with the model group, MDA decreased significantly in Allo group, howere, SOD increased significantly, it suggests that Allo can prompt antioxidant capacity and increase free radical scavengers.The results suggest that the effection of Allo on ventricular remodeling may be related to the removal of OFR.ET is one of the vasoconstrictor substances, NO is a vasoactive substances. They are secreted by endothelial cells, but they have opposite effects. ET can promote ventricular remodeling, howere, NO can prevent it The results showed that: Compared with the model group, the plasma ET is significantly reduced and NO is significantly increased in Allo group. It suggests that Allo may correct vascular endothelial dysfunction caused by myocardial ischemia reperfusion and improve the dysfunction of coronary artery endothelial cells to prevent MIR injury, thus inhibiting ventricular remodeling.In this experiment, it confirmed that allopurinol have a stronger anti-alcohol effect, blocking OFR generation; removing OFR directly; increasing antioxidant capacity, improving endothelial dysfunction.Conclusion: Allo can be effective in improving left ventricular remodeling, which can remove free radicals and improve vasodilation functions.
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