Study Of Ovain's Protection To Heart Muscle Isechemia In Rats Induced By Isoprenaline

The agents of the coronary atherosclerotic heart disease are the heart muscle ischemia and hypoxia, which could induce the disorder of the cardiac heart muscle contraction function, diastolic function and the dysbolism of the cardiac muscle cells as well. So coronary atherosclerotic heart disease could be called one of the metabolic diseases . Repetat heart muscle ischemia could induce the adaptabile changes of the myocardial metabolism.When the heart muscle is in the state of ischemia and hypoxia,the oxidative phosphorylation fuction of the heart muscle is down regulated and the heart muscles'function to synthesize novain is weakened, and the heart muscle will depend on the exogenous novin.Novain is one of the treatments to the coronary atherosclerotic heart disease.Novin can improve the energy metabolism of the heart muscles.The effect of the biological oxidation,clean the oxygen free radical and inhibit the apoptosis of the heart muscle, and then protect the heart muscles.The objective of the study is to discover the protection of novain to the myocardial ischemia rats by detecting the level of the malondialdehyde ,superoxide dismutase ,the Bcl-2 and the caspas-3. Methods1,Model and administration50 majority male rats weight (200±50)gram were divided into 3 groups.The control group (Ⅰgroup,n =10),the novain group(Ⅱgroup,n=20) and the trimetazidine group(Ⅲgroup,n=20).TheⅠgroup rats were intragastric administrationed 200 milligram sodium chloride every day, theⅡgroup rats were intraperitoneal injected novain by 200 milligram per kilogram every day and theⅢgroup rats were intragastric administrationed trimethazine by 5 milligram per kilogram every day.All the 3 groups mentioned were intraperitoneal injected 5 milligram aleudrin per kilogram on the 43rd day and 8.5 milligram aleudrin per kilogram on the 44th day and were executed on the 45th day.2 SpecimensEach rat was Weighed every week.On the 45th day,the rats were killed and the heart were kept in formalin.Before the heart was reciped, two ml blood was got from the heart and MDA and SOD were detected in the blood immediately.The treatment of the rat heart:Wash the blood out with the sodium chlodise and cut the tissue of the base of heart,the left atrium, right atrium and the right ventricle. The left ventricle of heart was indulged into 10% formaldehyde,by dehydrating,paraffin imbedding and cut into 2 um,then stained by normal pathology and immunohistochemistry stained.3 Statistical treatmentAll the datas were demonstrated as (±s) and analysised by SPSS 12.0,the group comparison was single factor analysis of variance. P<0.05 and P<0.01 was statistical significance.ResultsTheⅡgroup rats'weights increase fast after the 6th week of the experiment,while theⅠand theⅢrats'weight increase lower than theⅡgroup.Both theⅠand theⅢgroup appear deputer and move slower than theⅡgroup.There was two rats died in theⅠgroup andⅢgroup especially and one rat died in theⅡgroup.The level of MDA in the blood of theⅠgroup rats was higher and the level of SOD was lower than the other two groups.The level of SOD in theⅡgroup was higher than the other two groups'(P<0.01 and P<0.05).The level of SOD in the III was higher than the I group's. The pathology of the tissue:We find that the heart muscle cells were pyknosis,transverse striation,karyopycnosis and focal necrosis in the I group,the heart muscle cells were cloudy swelled,few inflammatory cells infiltrated,the blood vessels were congestioned,no cellular necrosis in theⅡgroup,and the cardiac muscle fibers were cloudy swelled significantly,the transverse striation were disappear,focal necrosis,and light inflammation in theⅢgroup.Stained by TUNEL:The apoptosis cells in theⅠgroup were the mostly in the 3 groups.The apoptosis cells in theⅡgroup were less than that in theⅠgroup(P<0.01),the apoptosis cells in theⅢwere less than theⅠgroup as well(P<05),but the difference between theⅡgroup and theⅢgroup was not significant(P>0.05).Immunohistochemistry analysis showed that the cells with Bcl-2 protein positive staining were more than before,while the cell with Bcl-2 protein positive staining in theⅡand theⅢwere more than the cells in theⅠgroup(P<0.01 and P<0.05).The cells with Caspase-3 protein positive staining were more than before and the cells with Caspase-3 protein positive staining were more than theⅠgroup, and the difference was significant.ConclusionIn the heart muscle ischemia rats models treated by aleudrin, Novain appears as follows:1. Novain can reduce the production of MDA and improve the activity of SOD which can clean the oxygen free radical.2. Novain can lessen the change of the heart muscle and reduce the focal necrosis caused by ischemic and hypoxia damage.3. Novain can reduce the apoptotic index of the heart cells.4. Novain can inhibit the breakdown of Bcl-2,the increasement of Caspase-3,and then reduce the apoptosis of the heart cells.