Association Of S100A4 Gene And Protein And E-cad Protein With Differentiation, Metastasis And Prognosis Of Colorectal Carcinoma

Objective To study the expression of S100A4 and E-cad protein in colorectal carcinoma and observe the expression of S100A4 gene in the colorectal carcinoma cell lines with different differentiation and explore association of S100A4 gene and protein and E-cad protein with differentiation, metastasis and prognosis of colorectal carcinoma in order to supply new target and theory for clinical prevention and cure of colorectal carcinoma. Methods The S-P immunohistochemical method was used to detect the expression of S100A4 and E-cadherin in 87cases with colorectal carcinoma and 87 cases with adjacent colorectal tissues and the expression of S100A4 in 51cases with colorectal adenoma; S-P immunocytochemical method was used to detect the expression of S100A4 in three colorectal carcinoma cell lines Caco-2, HT29, HCT116 with different differentiation;the mRNA expressions of S100A4 were measured in the colorectal carcinoma cell lines by using RT-PCR. Results 1. There was no expression of S100A4 in grandular epithelium of adjacent colorectal tissues. The positive expression rate of S100A4 was 12.0%(6/51) in colorectal adenoma. The positive expression rate of S100A4 was 64.4%(56/87) in colorectal carcinoma. There was significant difference among colorectal carcinoma and adjacent group, colorectal adenoma tissues (P<0.01). The expression of S100A4 was positively correlated with the clinical stages, lymph node metastasis and five-year survival (P<0.05), but not with other clinicopathologic factors (P>0.05). 2. S100A4 protein was expressed in the three colorectal carcinoma cell lines; the order of the expression of S100A4 mRNA was: HCT116 the highest, HT29 the second, Caco-2 was the lowest;there were significant differences in the expression level of S100A4 mRNA of the three cell lines(P<0.01). 3. There was 100.0% (87/87)expression for E-cadherin in adjacent colorectal tissues. The positive expression rate of E-cadherin was 62.1%(54/87)in colorectal carcinoma. There was significant difference between colorectal carcinoma and adjacent group(P<0.01). The expression of E-cadherin was positively correlated with the clinical stages, lymph node metastasis, pathological type, tumor site and five-year survival (P<0.05), but not with other clinicopathologic factors (P>0.05). 4. The expression of S100A4 was not significantly correlated with E-cadherin in colorectal carcinoma (r=-0.087, P>0.05). Conclusion 1.The expression of S100A4 could participate in colorectal carcinoma carcinogenesis and malignant progression, especially might play a role in progression of carcinoma and prognosis, and might be expected to become new biomarker and hidden target for curing colorectal carcinoma. 2. There was down regulation expression of E-cadherin in colorectal carcinoma and E-cadherin was closely related with colorectal cancer invasion, metastasis and prognosis. 3. S100A4 and E-cadherin play different roles in the invasion and metastasis of colorectal cancer.