IGF-â… R, IGF-â… and IGF-â…¡ Expression In Colorectal Cancer And Their Roles

Objective1. The aim of this study was to detect the expression of IGF-â… R, IGF-â… and IGF-â…¡in cancer tissues, neighboring cancer tissues, and normal tissues of colorectal cancer patients, and to find the expression difference among the three different tissues.2. To analyze the relationship between the expression of IGF-â… R, IGF-â… , IGF-â…¡and the clinic pathologic factors.3. To analyze the relationship of IGF-â… R expression with IGF-â… expression, IGF-â…¡expression, and to explore the possible mechanism of IGF system in the development of colorectal cancer.Methods1. Protopathic patients with colorectal cancer were selected in the study. All cases were confirmed by pathology and were chose from No.8 hospital in Wuhan. Through looking up the medical record of these patients, some information needed was listed in the questionnaire. The contents included demographic, clinical, pathological and some other features.2. The expression of IGF-â… R, IGF-â… and IGF-â…¡was analyzed by immunohistochemical SP method. The dilution gradient was designed according to the relative references, and the dilution was determined by the pilot study result.3. The positive cut off point value of each indicator was determined by relative references, the staining was interpreted by two investigators who were blind to the clinical and pathological data, and the intro- and inter-observer agreement was evaluated by the consistency rate and Kappa analysis.Results1. The positive rates of IGF-â… R, IGF-â… and IGF-â…¡expression in cancer tissue were significantly higher than in neighboring cancer tissue and normal tissue(P<0.05). The positive rates of IGF-â… R and IGF-â…¡expression in neibouring cancer tissues and normal tissues were not significantly different(P>0.05).2. There were no significant associations between IGF-â… R, IGF-â… , IGF-â…¡expression and clinic pathologic factors, except for the IGF-â… R expression and cancer location.3. The IGF-â… R expression and IGF-â… expression was not significantly different(P>0.05),so was IGF-â… R and IGF-â…¡(P=0.05) in cancer tissues. The Consistency of IGF-â… R expression and IGF-â… expression was relatively weak, so was IGF-â… R and IGF-â…¡(P<0.05).Conclusions1. The study only found that the expression rates of IGF-â… R, IGF-â… and IGF -â…¡were statistically significant among cancer tissues, neighboring cancer tissues, and normal tissues. The role of IGF system in colorectal cancer was not obvious in the study.2,The study did not find out the positive association between the IGF-â… R, IGF-â… and IGF -â…¡expression and the clinical pathology factors.The results need to be supported and demonstrated by further preclinical, clinical and population studies.3. The results of Correlation analysis suggested that paracrine, autocrine, and endocrine ways all played some roles in the development of colorectal cancer.