| Objective:(1 ) To explore the relationship between MBL serum level and GDM,and to observe the role of point mutation of MBL gene in GDM in order to learn the function of immune factors in GDM;(2) To select a good method to measure short gene point mutation.Methods:Gestational women in prenatal check in The first Affiliated Hospital of Anhui Medical University during Sep.2005 and Mar.2007 were screened and diagnosed in GDM.48 of them were diagnosed with GDM,which were set as GDM group.Among the normal women in the same time,48 were taken out to set as the normal comparison group.General statistics data was collected,including age,stature,weight,family history of diabetes etc.3-5ml venous blood was extracted from gestational women.The serum and cell components of the blood were separately preserved after centrifuged.Enzyme-linked immunosorbent assay was used to measure the MBL serum level of both groups while polymerase chain reaction -Single strand conformation polymorphism(PCR-SSCP) was used to measure the point mutation of the 54th codon of MBL gene.χ~2 est was used to analysis the relationship between the MBL serum level and GDM,the relationship between the MBL serum level and point mutation of the 54th codon of MBL gene,as well as the relationship between the point mutation of the 54th codon of MBL gene and GDM.Non-conditional logistic regression was used to learn the relationship between the MBL serum level and the point mutation of the 54th codon of MBL gene and GDM. Results:(1 ) The serum concentration of MBL in GDM group and normal group had an abnormal distribution.The MBL serum level was between 4.5μg/L and 36.4μg/L,and the average was 14.1±4.4μg/L.The average MBL serum level in GDM was 13.2±6.1μg/L, remarkably lower than that of normal group16.7±5.4μg/L(P＜0.05);(2) It was divided into two groups by the average of MBL serum of all 96 gestational women and the comparison showed that there was 31 women with GDM in low MBL serum level group,while only 6 normal women in this group.GDM morbidity in low MBL level group was evidently higher than the high level group.In GDM group,there was 31 women in low MBL level group,with 17 ones in high level group.There was a significant difference in the comparison of the two groups(χ=2.15,P＜0.05 );(3) The Logistic regression analysis showed that low MBL serum level(OR=6.5989, 95%CI=8.2430-54.6086),high progestation BMI(OR=4.3852,95%CI=7.3540-34.3561 ), family history of diabetes(OR=4.1254,95%CI=4.0247-27.2024) were all increased the risk of GDM(P＜0.01);(4) Three genotypes were found in PCR-SSCP:Among 96 samples,there were 82 normal ones and 14 mutational ones,with 11 mutational ones in GDM group(22.91%) and 3 mutational ones in normal group(6.25%).The mutational rate of MBL gene in GDM group was significantly higher than that of the normal,(5 ) The Logistic regression analysis showed that point mutation of MBL gene (OR=4.971,95%CI=1.094-4.041 ) increased the risk of GDM(P＜0.01);(6 ) There were 10 mutational samples in low MBL serum level group(29.4%).In high serum level group,there were 4 mutational samples(6.8%).The mutational rate of the former group was evidently higher than that of the latter.Among the point mutation women, there was 10 in low MBL serum level group,and 4 in high level group(χ~2=7.17,P＜0.01 ). Conclusions:(1 ) There is difference in MBL serum level between GDM group and the normal group.The study shows that the MBL serum level of GDM is lower than that of normal ones and low serum MBL level can increase the risk of GDM.(2) The point mutation of the 54th codon of MBL gene may lead to the low MBL serum level,and can increase the risk of GDM.This hints that the point mutation of the 54th codon of MBL gene may be one of risk factors of GDM.(3 ) According to the statistical analysis,the MBL serum level and gene point mutation of MBL may be the independent risk factors of GDM.(4) It is better to use PCR-SSCP in point mutation detection of short gene than other methods.
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