The Influence Of PPARγ Ligand 15-d-PGJ2 To The Ability Of JEG-3 Cell Lien's Invasion

ObjectiveThe infiltratration of Placenta nurse cell was the interactive function of changing between cell to cell and cell to base material.The element of ECM will be changed in this process,and adjusted and controled by cellfactor,growth factor,adhereing molecule,proteolytic ferment and many other factors.PPARγ,was discovered in recent years,and a member of nuclear hormone receptor superfamily,it locates in the crossing point of many signals transcribing,controling many cells from hyperplasy,invasion,differentiation,and apoptosis in the level of transcribing.After research,we find it that PPARγligand can inhibit tumour cell proliferating in neuroblastoma,glioblastom,prastatic carcinoma,and lung cancer cell,promoting tumour cell to differentiate and leading to death,PPARγligand plays a great part in resisting cell from proliferating.This experiment,based on cultivating chorioepithelioma cell line(JEG-3cell line)and detecting PPARγproteinum expression, applied the research of covered matrigel invasion model to study PPARγ,and the influnce of PPARγligand to the JEG-3 cell line's infiltrating ability,which provide evidence for further investigating PPARγ's effect in nutrient cell abnormal infiltration cyesis complication.Methodscell-cultivating technique helps to cultivate JEG-3 cell line,and fluorescence immunocytochemistry technique helps to indentify the expression of PPARγproteinum in JEG-3 cell line.With the help of covered matrigel extraorgan invasion model,we could observe the change of JEG-3 nutrient cell line's infiltrating ability intervened by PPARγexcitomotor 15-d-PGJ2 's different density,and identify if it is dependent on the density.ResultsThe result of Immune flourescence cytochemistry shows that the PPARγproteinic reacts in JEG-3 cell line,green flourescence masculine signal mainly locates in cell nucleus.Infiltrating experiment result shows that after JEG-3 cell line was handled by 15-d-PGJ2,its infiltrating ability obviously weakens.when 15-d-PGJ2's density are 0.1μmol/l,1μmol/l and 10μmol/l,cell Infiltrating index are 0.81±0.03,0.68±0.04 and 0.54±0.04 respectively.Comparing with the example(1.00±0.00),it has obvious statistical significance.And with the medicine density increases,JEG-3 cell line Infiltrating index descends correspondently,owing dose-dependent relation.ConclusionThrough immunoflourescence cytochemical methods,the reaction and location of PPARγin JEG-3's cell.The transwell cabin model mentioned above helps to detect the influence of PPARγand ligand 15-d-PGJ2 to the Infiltrating ability.Result shows that PPARγproteinum reacts in cultivated JEG-3 cell line,and mainly locates in cell nucleus; after being activated by 15-d-PGJ2,PPARγcan inhibit cell infiltrating.And with ligand density's increase,infiltrating ability descends gradually,also owing dose-dependent relation.The result above explains that after PPARγwas activated by ligand,it can negatively control nutrient cell from infiltrating.Because 15-d-PGJ2 is PPARγ's natural ligand,and exists in histiocyte naturally,we can suppose that,for some reason,in humans' early duration period of pregnancy,this kind of ligand increases on female foetus interface,which can certainly control nutrient cell's infiltratration,and vice versa.insufficient or excessive infiltrate both can cause abnomal pregnancy.