Study Of The Probe Effect Of LRP16 Gene In Endometriosis

Endometriosis is a hormone-dependent diseases,occurs in young and middle-aged women.Pathogenesis has not yet been fully clarified,for the majority of scholars to accept is endometrial planting theory.The new view is that endometrial cells with endometriosis patients grow,invasion and angiogenesis in the ectopic place like as cancer biology.Previous studies finding LRP16 gene is a kind of estrogen-dependent cancer-related genes,therefore,this study tried to explore the relationship about LRP16 gene and E-cadherin,ER, PR in the EMS development process,and may play a role.At the same time,this study observe F-actin through the use of the MCF'7 of breast cancer Cells,in an attempt to explain LRP16 in the EMS whether by changing the cytoskeleton protein F-actin to mediated the movement in endometrial cells.This study is divided into two parts:First Study on the expression of LRP16 and E-cadherin,ER,PR in endometrial and endometriotic tissues of patients with endometriosisObjective:To study the expression of LRP16 and E-cadherin,ER,PR in eutopic endometrium and ectopic endometrium of endometriosis.Methods:The expression of LRP 16 and E-cadherin,ER,PR in 72 cases of endometriosis patients was detected by immunohisto-chemistry methods.Results:The expression of LRP16 in epithelioglandular endochylema in eutopic endometrium of EM was significantly higher than that of ectopic endometrium(P＜0.05).It was no difference between in eutopic endometrium and in ectopic endometrium that expression of LRP16 in epithelioglandular nuculus.The expression of E-cad in ectopic endometrium of EM was higher than that of eutopic endometrium(P＜0.05).It was no significantly correlation between LRP16 and E-cadherin in eutopic and ectopic endometrium.The expression of ER,PR in epithelioglandular nuculus in eutopic endometrium of EM was higher than that of ectopic endometrium(P＜0.05). The expression of PR was higher than LRP16 in ectopic endometrium of EM(P＜0.05),The expression of ER was lowwer than LRP16 in ectopic endometrium of EM(P＜0.05).The expression of ER,PR was lowwer than LRP16 in eutopic endometrium of EM(P＜0.05).Conclusion:Expression of LRP16,ER,PR,E-cadherin in epithelioglandular endochylema were difference in ectopic and eutopic endometrium of EM,these abnomal expression may be correlate with progress of endometriosis.Second To study the expression about F-actin in three expression different LRP16 gene of cell linesObjective:To study the expression of the F-actin in LRP16 protein expression of the three different cell lines,MCF-7 cell lines,MPL-374 cell lines and MPL-GFPi cell lines,then further analysis the relationship about between LRP16 protein and F-actin in cell migration.Methods:Using immunofluorescence method.F-actin with specific FITC-phalloidin fluorescent dye,the three cells were observed between the three different cell.Results:in the MPL-374 cells which be tagged off by FITC-halloidin,LRP16 gene expression inhibition rate of 90 percent,LRP16 gene expression for the MPL-GFPi cells were O,Membrane inside the cells and the cells of drawing burr-like processes,the more visible green fluorescent.For stability of expression LRP16 gene of MCF-7 cell,the cell membrane inside and the cells of drawing burr-like processes,green fluorescent significantly was be weakenedConclusion:The high expression of LRP16 gene can promote the F-actin depolymerization,and changing F-actin into the G-actin.Three cells were not significantly different,that can speculate F-actin depolymerization not enough to cause the imbalance ein the MCF-7 cells within the G-actin and F-actin into belance.the LRP16 cell gene high expression of MCF-7 lead to change the F-actin into the G-actin,the changes arising from the change of drawing,may lead to changes in the interaction between cells,thus enabling cells prone to migration.Conclusion: 1,LRP16 in the nucleus and cytoplasm is expressed at the same time,and ectopic endometrial LRP16 cytoplasm of the reign of less than endometrial and reign in the cytoplasm of endometrial expression than in the nucleus.2,The expression of LRP16 in epithelioglandular endochylema in ectope endometrium of EMS was significantly higher than that E-cadherin,there is no different in eutopic endometrium and the expression of LRP16 in epithelioglandular nucleus.The expression of E-cadherin in ectope endometrium was significantly higher than that in eutopic endometrium,so,the relation about LRP16 and E-cadherin tends to negative correlation.The different expression of ER,PR and LRP16 mey be cue the relation in among of them.3,the LRP16 cell gene high expression of MCF-7 lead to change the F-actin into the G-actin,the changes arising from the change of drawing,may lead to changes in the interaction between cells,thus enabling cells prone to migration.