Study Correlation Of HBV Genotype With Clinical Characteristics Of Hepatocellular Carcinoma(HCC) And Expression Of VEGF And VEGFR (Flt-1 And Flk-1) In HCC Issue

Objective:To investigate the correlation of HBV Genotype and clinical characteristics of HCC(hepatocellular carcinoma)in north of China.Methords: HBV Genotype,HBVDNA level,HBeAg positive rate,tumor lesions, pathological grading,survival time,AFP,liver injury and clinical date of HCC were analyzed retrospectively.Result:(1)In the 156 patient with HCC,20 were HBV Genotype B,134 were HBV Genotype C and 2 were HBV Genotype Non-BC.(2)HBV Genotype B patients were younger than that of Genotype C (p＜0.01).The level of ALT,AST,TBIL,HBVDNA and HBeAg positive rate of HBV Genotype C were higher than that of Genotype B(p＜0.01),and Genotype C seemed closely related to HCC in north of china.(3)The single lesion was common in Genotype B patients with HCC,but mutiple lesions were often found in Genotype C patients with HCC(p＜0.01).The tumor pathology classification have no difference between HBV Genotype B and HBV Genotype C.1 and 2 years survival rate of HBV Genotype B was 84%and 69%,respectively.The latter of HBV Genotype B was higher than that of HBV Genotype C(p＜0.05).(4)The positive rate of AFP was 70%and 81.34%in HCC of HBV Genotype B and HBV Genotype C respectively.Conclusion:(1)HBV Genotype C seemed closely related to HCC in north of china.The average age of HBV Genotype B were younger than that of Genotype C in HCC.(2)The liver injured of HBV Genotype C was more severe than that of HBV Genotype B.Single lesion was common in Genotype B HCC patients.Survival times of patients with HCC was longer than that of HBV Genotype C.(3)AFP was still significant to diagnosis and evaluate the effect whether in HBV Genotype B or HBV Genotype C. Objective:To analyze the expression of VEGF(vascular endothelial growth factor),and VEGFR(Flt-1 and Flk-1)protein in liver tissue of HCC and their relation to tumor pathology classification,investigate the correlation between the expression of those and HBV Genotypes in patients with HCC.Methods:All samples were studyed in an immunohistochemistry method to analyze the expression of VEGF,Flt-1 and Flk-1.Results:(1)In liver tumor tissues,VEGF, Flt-1 and Flk-1 were mainly located in the cytoplasm of hepatoma cells,vascular endothelial cells and hepatic sinusoid cells.They were expressed highly, demonstrated apparent higher positive rates of 83.3%,76.7%,80%respectively, compared with those in HBV-cirrhosis and chronical hepatitis B(p＜0.01).(2)The expression of VEGF protein in poorly differentiated hepatocellular carcinoma was higher than in well differentiated hepatocellular carcinoma(p＜0.01).(3)There was no obvious difference of positive rates of VEGF,Flt-1 and Flk-1 expression among various HBV Genotypes(p＜0.05).However,the VEGF protein expression score in HBV Genotype C hepatocellular carcinoma was evidently higher than it in HBV Genotype B(p＜0.05).Conclusion:(1)The expression of VEGF,Flt-1 and Flk-1 in HCC were significantly higher than in cirrhosis and CHB.(2)There was a positive correlation between the expression of VEGF,Flt-1,Flk-1 and the expression of carcinoma malignant degree.(3)The high level expression of VEGF around the necrotic tumor tissues may be biological basis of cancer recurrence or metastasis after receiving treatments which blocking blood supply is the main therapy.(4)It deserves attention that whether the high level expression of VEGF in patients with HBV Genotype C hepatocellular carcinoma is one factor effecting the prognosis.