Electrochemical Investigation Of Drugs Interacting With Protein And The Oxygen Reduction In The Solution At The Chemically Modified Electrode

The antibiotic medicament Ceftriaxone ( CTRX, trade name Jun Bi Zhi) is very popular with clinic due to its controlled release, broad spectrum, high antisepsis and little side reaction activities. A sensitive method for the electrochemical behavior of ceftriaxone and the determination of sodium ceftriasonate by cyclic voltammetry was proposed. The interactions between CPFX, AA and bovine serum albumin (BSA) were also studied by electrochemical method. Moreover, because of the biological compatibility of the titanate nanotube, we determined its electrochemical behabior on the chemically modified electrode and electrocatalytic reduction to oxygen dissolved in solution. The main contents are as follows:Chapter 1. The electrochemical behavior and the determination of sodium ceftriasonate by cyclic voltammetryA sensitive method for the electrochemical behavior of ceftriaxone and the determination of sodium ceftriasonate by cyclic voltammetry was proposed. In an KCl solution of pH 2.31, a reductive peak was observed at -1000mV (vs.SCE) and its peak current kept a linear relationship with the concentration of sodium ceftriasonate in the range of 4.0×10^-6～2.5×10^-4, the standard deviation of the result obtained was 0.44 with the average recoveries of 98.33%. This method was simple and accurate.Chapter 2. Investigation on the interactions of ciprofloxacin and Ascorbic acid with bovine serum albumin①The interactions between Ascorbic acid and bovine serum albumin (BSA) were studied using cyclic voltammetry under physiological condition: pH 7.30, 0.1 mol/L NaCl solution.The results showed that the electrochemically inactive supramolecular structures were formed in BSA-AA complexes.The binding constant is 1.38×107 L/mol , and the number of binding site is 2.②First, an electrochemical investigation of the interaction of ciprofloxacin with bovine serum albumin (BSA) is presented, using a glassy carbon electrode in HAc-NaAc buffer solution at pH 4.74, which indicates that an electrochemially inactive supramolecular complex BSA-ciprofloxacin was formed. Other, A new pH sensor, ciprofloxacin modified glassy carbon electrode was prepared. The parameters such as the range of pH response, the slope of pH sensing, stability et al were studied.Chapter 3. The electrocatalytic reduction of oxygen dissolved in soulution on the titanate nanotubes modified electrodeThe modified electrode coated with titanate nanotubes was used to catalyze the reduction of oxygen dissolved in solution, contrast to bare GC, this prepared modified electrode exhibited a higher catalytic activity. The reduction potential shifted to the positive direction and the reduction current increased significantly on the modified electrode.